公开(公告)号：US20210108217A1

公开(公告)日：2021-04-15

申请号：US16998803

申请日：2020-08-20

Applicant: Monsanto Technology LLC

Inventor： Changlin Fu ,  Jinyuan Liu

IPC: C12N15/82 ,  C12N15/66

Abstract: The current invention provides a modular vector system that enables the insertion of gene expression cassettes in a recursive directional stacking fashion by rare restriction sites which requires only one type of vector. The invention also provides DNA molecules, compositions, and transgenic organisms, plants, plant tissues, plant seeds, and cells comprising recombined restriction sites for rare restriction enzymes.

公开(公告)号：US20150152428A1

公开(公告)日：2015-06-04

申请号：US14405754

申请日：2013-06-10

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Changlin Fu ,  Jinyuan Liu

IPC: C12N15/82 ,  C12N15/66

Abstract: The current invention provides a modular vector system that enables the insertion of gene expression cassettes in a recursive directional stacking fashion by rare restriction sites which requires only one type of vector. The invention also provides DNA molecules, compositions, and transgenic organisms, plants, plant tissues, plant seeds, and cells comprising recombined restriction sites for rare restriction enzymes.

Abstract translation: 本发明提供了一种模块化载体系统，其能够以仅需要一种载体的稀有限制性位点以递归方向堆叠方式插入基因表达盒。 本发明还提供了DNA分子，组合物和转基因生物，植物，植物组织，植物种子和包含稀有限制酶重组限制性位点的细胞。

公开(公告)号：US10829772B2

公开(公告)日：2020-11-10

申请号：US14405754

申请日：2013-06-10

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Changlin Fu ,  Jinyuan Liu

IPC: C12N15/00 ,  C12N15/82 ,  C12N15/66

Abstract: The current invention provides a modular vector system that enables the insertion of gene expression cassettes in a recursive directional stacking fashion by rare restriction sites which requires only one type of vector. The invention also provides DNA molecules, compositions, and transgenic organisms, plants, plant tissues, plant seeds, and cells comprising recombined restriction sites for rare restriction enzymes.