公开(公告)号：US20170130254A1

公开(公告)日：2017-05-11

申请号：US15127373

申请日：2014-03-26

Applicant: NANJING UNIVERSITY OF TECHNOLOGY

Inventor： Hanjie YING ,  Zhi CAO ,  Yong CHEN ,  Xiaochun CHEN ,  Jinglan WU ,  Jingjing XIE

IPC: C12P19/30

CPC classification number: C12P19/30 ,  C12Y301/11005

Abstract: A nucleotide production process comprises: decomposing an RNA by using a nuclease P1 so as to obtain nucleotides AMP, GMP, CMP and UMP, converting part or all of the nucleotide AMP into a nucleotide IMP by using adenosine deaminase, separating the obtained nucleotide by using an ion exchange resin, and then performing concentration and crystallization to obtain purified nucleotides AMP, GMP, CMP, UMP and IMP or obtain purified nucleotides GMP, CMP, UMP and IMP. The whole biocatalysis production of nucleotides is implemented by using a double-enzyme catalysis method, and high-purity nucleotides are obtained by using an ion resin separation technology and a solvent crystallization method; and the production process is simple and environmentally-friendly, and has low costs, high product safety and purity.

公开(公告)号：US20160032272A1

公开(公告)日：2016-02-04

申请号：US14651071

申请日：2012-12-13

Applicant: NANJING UNIVERSITY OF TECHNOLOGY

Inventor： Hanjie YING ,  Yong CHEN ,  Qingguo LIU ,  Jinglan WU ,  Xiaochun CHEN ,  Jingjing XIE

IPC: C12N11/08 ,  C12P7/06

CPC classification number: C12N11/08 ,  C12N11/02 ,  C12N11/12 ,  C12P7/06 ,  D06M13/123 ,  D06M13/332 ,  D06M13/418 ,  D06M15/59 ,  Y02E50/17

Abstract: Provided is a preparation method of a yeast cell immobilization medium, which comprises the following steps: (1) boiling a fiber material in boiling water and drying the fiber material; (2) soaking the fiber material in a surface modified aqueous solution with a concentration of 1-100 g/L, using hydrochloric acid to adjust a PH of the solution to 7.0, fully rinsing the fiber material in deionized water and drying the fiber material; (3) soaking the fiber material in a cross-linking agent aqueous solution with a concentration of 1-100 g/L, fully rinsing the fiber material in deionized water and drying the fiber material; and (4) attaching the fiber material to supporting framework. Also provided is the yeast cell immobilization medium prepared using the preparation method and a method for producing ethanol using the yeast cell immobilization medium.

Abstract translation: 提供酵母细胞固定培养基的制备方法，其包括以下步骤：（1）在沸水中煮沸纤维材料并干燥纤维素材料; （2）将纤维材料浸泡在浓度为1-100g / L的表面改性水溶液中，使用盐酸将溶液的PH调节至7.0，将去离子水中的纤维材料充分漂洗并干燥纤维材料 ; （3）将纤维材料浸泡在浓度为1-100g / L的交联剂水溶液中，在去离子水中充分漂洗纤维材料并干燥纤维材料; 和（4）将纤维材料连接到支撑框架上。 还提供了使用制备方法制备的酵母细胞固定培养基和使用酵母细胞固定培养基生产乙醇的方法。