公开(公告)号：US20140051585A1

公开(公告)日：2014-02-20

申请号：US13968302

申请日：2013-08-15

Applicant: NATERA, INC.

Inventor： Dennis Prosen ,  Johan Baner ,  Nathan Hunkapiller ,  Matthew Hill ,  Bernhard Zimmermann

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00722 ,  C12N15/1093

Abstract: Embodiments include methods, compositions, and kits for creating genetic libraries useful for massively parallel genetic sequencing. Some embodiments are directed to methods of preventing the contamination of genetic libraries with material generated during the formation of other genetic libraries. In some embodiments, the methods employ adapters comprising universal priming sites. The methods can employ non-ligatable primers to generate non-ligatable amplification products so as to prevent unwanted ligation to adapters. In some embodiments, the non-ligatable primers contain uracil. Genetic material can be treated with uracil N glycosylase to prevent the unwanted ligation of uracil containing amplicons to adapters used for creating a second genetic library.

Abstract translation: 实施方案包括用于产生用于大规模并行遗传测序的遗传图谱的方法，组合物和试剂盒。 一些实施方案涉及防止在形成其他遗传文库期间产生的材料污染遗传文库的方法。 在一些实施方案中，所述方法使用包含通用引发位点的衔接头。 所述方法可以使用不可连接的引物来产生不可连接的扩增产物，以防止对适配器的不必要的连接。 在一些实施方案中，不可连接的引物含有尿嘧啶。 可以用尿嘧啶N糖基化酶处理遗传物质，以防止含有尿嘧啶的扩增子与用于产生第二遗传文库的适配器的不希望的连接。