公开(公告)号：US20230355766A1

公开(公告)日：2023-11-09

申请号：US17774695

申请日：2020-09-04

Applicant: NATIONAL CENTER FOR NANOSCIENCE AND TECHNOLOGY

Inventor： Baoquan Ding ,  Shaoli Liu ,  Qiao Jiang

IPC: A61K39/00 ,  A61P37/04

CPC classification number: A61K39/464499 ,  A61P37/04 ,  A61K2039/53 ,  A61K2039/55561 ,  B82Y5/00

Abstract: Provided are a DNA nanovaccine, a preparation method therefor and the use thereof. The DNA nanovaccine comprises a DNA nanostructure, a tumor antigen polypeptide-DNA complex and an immunologic adjuvant, and the immunologic adjuvant comprises a double-stranded RNA immunologic adjuvant and/or a CpG immunologic adjuvant. In the present invention, a nanostructure is constructed, wherein the nanostructure is assembled from a DNA template, a DNA chain for assisting in folding and a capture DNA chain. By hybridizing the capture DNA chain with a functional component, the precise positioning and assembling of a tumor antigen molecule and an immunologic adjuvant molecule on the surface of the DNA self-assembled nanostructure is realized; in addition, a controllable DNA molecule “switch” is designed on one side of the tubular DNA nanostructure, which switch can respond to the acid environment of an endosome after entering an antigen-presenting cell, and open the tubular structure responsively to release the tumor antigen and the immunologic adjuvant molecule. The nanostructure has a tumor antigen-specific immunostimulatory effect and is a tumor vaccine used for the immunotherapy and prevention of various types of malignant tumors.