公开(公告)号：US20150338396A1

公开(公告)日：2015-11-26

申请号：US14419804

申请日：2013-08-09

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： Hisashi NARIMATSU ,  Atsushi KUNO ,  Yuzuru IKEHARA ,  Yasuhiro HASHIMOTO ,  Keiro SHIROTANI ,  Kiyomitsu NARA ,  Yoshinobu KARIYA ,  Hiromi ITO ,  Kyoka HOSHI

IPC分类号： G01N33/53 ,  G01N33/536

CPC分类号： G01N33/5308 ,  G01N33/536 ,  G01N33/6854 ,  G01N2333/42 ,  G01N2333/4724 ,  G01N2400/00 ,  G01N2440/38 ,  G01N2496/00

摘要： It is intended to develop and provide a method for detecting a particular glycan-isoform rapidly and specifically by a small number of steps. The present invention provides a glycan-isoform detection method comprising quantifying an immune complex formed by the mixing of a test sample with a sugar chain non-reducing terminal residue-binding lectin and an antibody specifically binding to the protein moiety of the glycan-isoform, etc., comparing the obtained amount of the immune complex with the amount of a control immune complex obtained when a control sample is not mixed with the sugar chain non-reducing terminal residue-binding lectin or is mixed with a control protein, and determining the presence or absence of the glycan-isoform of interest in the test sample on the basis of the difference between these amounts.

摘要翻译： 旨在开发并提供一种通过少量步骤快速且具体地检测特定聚糖同种型的方法。 本发明提供了一种聚糖同种型检测方法，其包括定量通过将测试样品与糖链非还原性末端残基结合凝集素和特异性结合聚糖同种型蛋白质部分的抗体混合形成的免疫复合物， 将获得的免疫复合物的量与对照样品不与糖链非还原末端残基结合凝集素混合或与对照蛋白混合时获得的对照免疫复合物的量进行比较， 基于这些量之间的差异，测试样品中存在或不存在感兴趣的聚糖同种型。