公开(公告)号：US20220135931A1

公开(公告)日：2022-05-05

申请号：US17503186

申请日：2021-10-15

Applicant: Novartis AG ,  The Trustees of the University of Pennsylvania

Inventor： Fabio FACHIN ,  Rodney RIETZE ,  Michael R. GREENE ,  Lan CAO

IPC: C12M1/00 ,  B03C1/30 ,  B03C1/033 ,  B03C1/28 ,  B03C1/01 ,  C07K16/28 ,  C12M1/12 ,  C12N13/00

Abstract: The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.

公开(公告)号：US20190203230A1

公开(公告)日：2019-07-04

申请号：US16331967

申请日：2017-09-28

Applicant: NOVARTIS AG

Inventor： Fabio FACHIN ,  Michael FIORINO ,  Cameron LEE ,  Yi YANG ,  Jonathan YEN

IPC: C12N15/90 ,  C12N15/87

CPC classification number: C12N15/907 ,  C12N15/102 ,  C12N15/87 ,  C12N2310/20 ,  C12N2800/80

Abstract: In one aspect, a method of processing a cell is disclosed, which includes passing a cell through a pore of a membrane comprising a plurality of pores while exposing the cell to an agent so as to cause a change in the cell, thereby allowing said agent to enter the cell, where each of said pores extends from an input opening to an output opening and has at least one cross-sectional dimension, and in many embodiments a maximum cross-sectional dimension, less than a diameter of said cell. For example, at least one cross-sectional dimension of the pore, and in many embodiment the maximum cross-sectional dimension of the pore, can be less than about 40 microns, or less than about 30 microns, or less than about 20 microns, or less than about 15 microns, or less than about 10 microns.