公开(公告)号：US20140287475A1

公开(公告)日：2014-09-25

申请号：US14255642

申请日：2014-04-17

Applicant: Novozymes A/S ,  Novozymes, Inc.

Inventor： Howard Brody ,  Suchindra Maiyuran ,  Hiroaki Udagawa

IPC: C12P1/02

CPC classification number: C12P1/02 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.

Abstract translation: 本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）将丝状真菌菌株的双链可转录核酸构建体插入基因组中，所述双链可转录核酸构建体包含第一核苷酸序列，其包含 可操作地连接到靶基因的同源编码区的启动子和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二核苷酸 序列相对于第一个核苷酸序列处于相反方向; 和（b）通过在有利于产生干扰RNA的条件下培养丝状真菌菌株诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。 本发明还涉及丝状真菌菌株以及在这种丝状真菌菌株中生产目的生物物质的方法。

公开(公告)号：US09598698B2

公开(公告)日：2017-03-21

申请号：US14421330

申请日：2013-08-16

Applicant: NOVOZYMES A/S

Inventor： Howard Brody ,  Evee Ricacho ,  Hiroshi Teramoto

IPC: C12N15/80 ,  C12N15/113 ,  C12N15/10

CPC classification number: C12N15/80 ,  C12N15/1086 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/12 ,  C12N2330/51

Abstract: The present invention relates to methods for co-silencing expression of genes in a filamentous fungal strain by transitive RNA interference. The present invention also relates to methods for identifying a gene encoding a biological substance of interest.

公开(公告)号：US20160002646A1

公开(公告)日：2016-01-07

申请号：US14421330

申请日：2013-08-16

Applicant: NOVOZYMES A/S

Inventor： Howard Brody ,  Evee Ricacho ,  Hiroshi Teramoto

IPC: C12N15/80 ,  C12N15/10

CPC classification number: C12N15/80 ,  C12N15/1086 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/12 ,  C12N2330/51

Abstract: The present invention relates to methods for co-silencing expression of genes in a filamentous fungal strain by transitive RNA interference. The present invention also relates to methods for identifying a gene encoding a biological substance of interest.

Abstract translation: 本发明涉及通过传递性RNA干扰共同沉默丝状真菌菌株中基因表达的方法。 本发明还涉及用于鉴定编码感兴趣的生物物质的基因的方法。