公开(公告)号：US09476087B2

公开(公告)日：2016-10-25

申请号：US14326215

申请日：2014-07-08

申请人： Nanyang Technological University

发明人： Bengang Xing ,  Xianfeng Huang ,  Tingting Jiang ,  Rongrong Liu

IPC分类号： C12Q1/34 ,  C07D501/04 ,  C07D501/18 ,  C07D501/16 ,  C07D519/06

CPC分类号： C12Q1/34 ,  C07D501/04 ,  C07D501/16 ,  C07D501/18 ,  C07D519/06 ,  G01N2333/986

摘要： The present invention relates to compounds for and a method of detecting beta-lactamase activity in a sample. The sample is contacted with a nanoparticulate tag. The nanoparticulate tag comprises a metal or a combination of metals, or it comprises a nanotube of a metal, boron nitride and/or carbon. The respective metal is capable of forming one of a covalent bond, a coordinative bond and a non-covalent interaction with a thio or a seleno group. The sample is contacted with a compound of one of general formulas (I)-(III) and (VII)-(IX). At least one beta-lactam moiety of the compound is cleaved by the beta-lactamase activity in the sample. As a result a cleavage moiety Z-A-Z, Z-A-Z—R15, Z-A-Z—R16, Z-A-Z—R17, Z-A-Z—R18 or Z-G-N(R8)R9 is released that is immobilized on the surface of the nanoparticulate tag by a covalent bond via a Z atom. The presence of beta-lactamase activity is determined based on the presence of the cleavage moiety immobilized onto the surface of the nanoparticulate tag.

摘要翻译： 本发明涉及用于检测样品中β-内酰胺酶活性的化合物和方法。 样品与纳米颗粒标签接触。 纳米颗粒标签包括金属或金属的组合，或者它包括金属，氮化硼和/或碳的纳米管。 相应的金属能够形成共价键，配位键和与硫代或硒基的非共价相互作用之一。 将样品与通式（I） - （III）和（VII） - （IX）之一的化合物接触。 化合物的至少一个β-内酰胺部分被样品中的β-内酰胺酶活性切割。 结果，通过Z原子通过共价键将解离部分Z-A-Z，Z-A-Z-R15，Z-A-Z-R16，Z-A-Z-R17，Z-A-Z-R18或Z-G-N（R8）R9释放固定在纳米颗粒标签的表面上。 基于固定在纳米颗粒标签表面上的切割部分的存在来确定β-内酰胺酶活性的存在。

公开(公告)号：US20140322739A1

公开(公告)日：2014-10-30

申请号：US14326215

申请日：2014-07-08

申请人： Nanyang Technological University

发明人： Bengang Xing ,  Xianfeng Huang ,  Tingting Jiang ,  Rongrong Liu

IPC分类号： C12Q1/34 ,  C07D501/16 ,  C07D501/04 ,  C07D519/06

CPC分类号： C12Q1/34 ,  C07D501/04 ,  C07D501/16 ,  C07D501/18 ,  C07D519/06 ,  G01N2333/986

摘要： The present invention relates to compounds for and a method of detecting beta-lactamase activity in a sample. The sample is contacted with a nanoparticulate tag. The nanoparticulate tag comprises a metal or a combination of metals, or it comprises a nanotube of a metal, boron nitride and/or carbon. The respective metal is capable of forming one of a covalent bond, a coordinative bond and a non-covalent interaction with a thio or a seleno group. The sample is contacted with a compound of one of general formulas (I)-(III) and (VII)-(IX). At least one beta-lactam moiety of the compound is cleaved by the beta-lactamase activity in the sample. As a result a cleavage moiety Z-A-Z, Z-A-Z—R15, Z-A-Z—R16, Z-A-Z—R17, Z-A-Z—R18 or Z-G-N(R8)R9 is released that is immobilized on the surface of the nanoparticulate tag by a covalent bond via a Z atom. The presence of beta-lactamase activity is determined based on the presence of the cleavage moiety immobilized onto the surface of the nanoparticulate tag.

摘要翻译： 本发明涉及用于检测样品中β-内酰胺酶活性的化合物和方法。 样品与纳米颗粒标签接触。 纳米颗粒标签包括金属或金属的组合，或者它包括金属，氮化硼和/或碳的纳米管。 相应的金属能够形成共价键，配位键和与硫代或硒基的非共价相互作用之一。 将样品与通式（I） - （III）和（VII） - （IX）之一的化合物接触。 化合物的至少一个β-内酰胺部分被样品中的β-内酰胺酶活性切割。 结果，通过Z原子通过共价键将解离部分Z-A-Z，Z-A-Z-R15，Z-A-Z-R16，Z-A-Z-R17，Z-A-Z-R18或Z-G-N（R8）R9释放固定在纳米颗粒标签的表面上。 基于固定在纳米颗粒标签表面上的切割部分的存在来确定β-内酰胺酶活性的存在。