公开(公告)号：US20060112447A1

公开(公告)日：2006-05-25

申请号：US10525318

申请日：2003-08-26

申请人： Natalia Bogdanova ,  Charles Romano

发明人： Natalia Bogdanova ,  Charles Romano

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  A01H1/00

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The present invention describes compositions and methods that are useful in the control of lepidopteran insect pests, and more particularly describes nucleotide sequences for use in plants that encode full-length and truncated insecticidal toxins, as well as chimeric toxins. The nucleotide sequences of the present invention exhibit modifications that, when compared to the native sequences obtained from Bacillus thuringiensis species, make them particularly useful for enhanced, improved, and or optimized expression in monocot and dicot plant species. Using methods well known to those skilled in the art the nucleotide sequences described herein can be used to transform plant cells and plant tissue in order to produce transgenic plants that express the encoded proteins, therefore conferring upon the transgenic plants the ability to resist insect infestation.

摘要翻译： 本发明描述了可用于控制鳞翅目昆虫害虫的组合物和方法，更具体地描述了用于编码全长和截短的杀虫毒素的植物以及嵌合毒素的核苷酸序列。 本发明的核苷酸序列表现出修饰，当与从苏云金芽孢杆菌属物种获得的天然序列比较时，使它们特别可用于单子叶植物和双子叶植物物种中增强，改良和/或优化的表达。 使用本领域技术人员熟知的方法，本文所述的核苷酸序列可用于转化植物细胞和植物组织，以便产生表达编码蛋白质的转基因植物，从而赋予转基因植物抵抗昆虫侵袭的能力。