-
公开(公告)号：US5399668A
公开(公告)日：1995-03-21
申请号：US888364
申请日：1992-05-26
IPC分类号： C07K14/415 , C07K19/00
CPC分类号： C07K14/415 , C07K2319/02
摘要： A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.
摘要翻译： 通过聚合酶链反应（PCR），使用对应于作为引物的两个区域的混合寡核苷酸和作为模板的三叶草胶体cDNA文库，分离编码水解蛋白的cDNA克隆（HEV1）。 HEV1长1018个核苷酸，包括204个氨基酸的开放阅读框。 推测的氨基酸序列含有17个氨基酸残基的随机信号序列，随后是187个氨基酸的多肽。 氨基末端区域（43个氨基酸）与外源蛋白相同，并显示与几丁质结合蛋白质和马铃薯和杨树中伤口诱导基因的氨基末端的同源性。 多肽的羧基末端部分（144个氨基酸）与马铃薯的伤口诱导基因的羧基末端区域同源74-79％。 伤口以及植物激素脱落酸和乙烯的应用导致叶片，茎和胶乳中hevein转录物的积累，但不能在根中积累，如使用cDNA作为探针所示。 在本发明的蛋白质的大肠杆菌中产生融合蛋白，由大肠杆菌产生的麦芽糖结合蛋白。
-
公开(公告)号：US5900480A
公开(公告)日：1999-05-04
申请号：US888366
申请日：1992-05-26
IPC分类号： C07K14/415 , C07H21/04
CPC分类号： C07K14/415 , C07K2319/02
摘要： A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.
摘要翻译： 通过聚合酶链反应（PCR），使用对应于作为引物的两个区域的混合寡核苷酸和作为模板的三叶草胶体cDNA文库，分离编码水解蛋白的cDNA克隆（HEV1）。 HEV1长1018个核苷酸，包括204个氨基酸的开放阅读框。 推测的氨基酸序列含有17个氨基酸残基的随机信号序列，随后是187个氨基酸的多肽。 氨基末端区域（43个氨基酸）与外源蛋白相同，并显示与几丁质结合蛋白质和马铃薯和杨树中伤口诱导基因的氨基末端的同源性。 多肽的羧基末端部分（144个氨基酸）与马铃薯的伤口诱导基因的羧基末端区域同源74-79％。 伤口以及植物激素脱落酸和乙烯的应用导致叶片，茎和胶乳中hevein转录物的积累，但不能在根中积累，如使用cDNA作为探针所示。 在本发明的蛋白质的大肠杆菌中产生融合蛋白，由大肠杆菌产生的麦芽糖结合蛋白。
-
公开(公告)号：US5187262A
公开(公告)日：1993-02-16
申请号：US587071
申请日：1990-09-24
IPC分类号： C07K14/415
CPC分类号： C07K14/415 , C07K2319/02
摘要： A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a puGOVERNMENT RIGHTSThis application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.
摘要翻译： 通过聚合酶链反应（PCR），使用对应于作为引物的两个区域的混合寡核苷酸和作为模板的三叶草胶体cDNA文库，分离编码水解蛋白的cDNA克隆（HEV1）。 HEV1长1018个核苷酸，包括204个氨基酸的开放阅读框。 推测的氨基酸序列含有17个氨基酸残基的随机信号序列，随后是187个氨基酸的多肽。 氨基末端区域（43个氨基酸）与外源蛋白相同，并显示与几丁质结合蛋白质和马铃薯和杨树中伤口诱导基因的氨基末端的同源性。 多肽的羧基末端部分（144个氨基酸）与马铃薯的伤口诱导基因的羧基末端区域同源74-79％。 伤口以及植物激素脱落酸和乙烯的应用导致叶片，茎和胶乳中hevein转录物的积累，但不能在根中积累，如使用cDNA作为探针所示。 在本发明的蛋白质的大肠杆菌中产生融合蛋白，由大肠杆菌产生的麦芽糖结合蛋白。
-
公开(公告)号：US6083687A
公开(公告)日：2000-07-04
申请号：US888367
申请日：1992-05-26
IPC分类号： C07K14/415 , C12Q1/68
CPC分类号： C07K14/415 , C07K2319/02
摘要： A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.
摘要翻译： 通过聚合酶链反应（PCR），使用对应于作为引物的两个区域的混合寡核苷酸和作为模板的三叶草胶体cDNA文库，分离编码水解蛋白的cDNA克隆（HEV1）。 HEV1长1018个核苷酸，包括204个氨基酸的开放阅读框。 推测的氨基酸序列含有17个氨基酸残基的随机信号序列，随后是187个氨基酸的多肽。 氨基末端区域（43个氨基酸）与外源蛋白相同，并显示与几丁质结合蛋白质和马铃薯和杨树中伤口诱导基因的氨基末端的同源性。 多肽的羧基末端部分（144个氨基酸）与马铃薯的伤口诱导基因的羧基末端区域同源74-79％。 伤口以及植物激素脱落酸和乙烯的应用导致叶片，茎和胶乳中hevein转录物的积累，但不能在根中积累，如使用cDNA作为探针所示。 在本发明的蛋白质的大肠杆菌中产生融合蛋白，由大肠杆菌产生的麦芽糖结合蛋白。
-
-
-
搜索结果
4 条记录 (耗时 0.018 秒)