公开(公告)号：US09429584B2

公开(公告)日：2016-08-30

申请号：US14770864

申请日：2014-02-27

申请人： National Cancer Center

发明人： Yasuhiro Matsumura ,  Masahiro Yasunaga ,  Yohei Hisata

IPC分类号： G01N33/86 ,  C07K16/18 ,  A61K47/48 ,  C07K16/36

CPC分类号： G01N33/86 ,  A61K47/6843 ,  C07K16/18 ,  C07K16/36 ,  C07K2317/33 ,  C07K2317/34 ,  G01N2333/75 ,  G01N2800/226

摘要： With the intention of providing an antibody which does not bind to fibrinogen and which has a high affinity for and a high specificity to insoluble fibrin, it is found that a site comprising the amino acids at positions 231 to 246 of the fibrinogen Bβ chain and a site comprising the amino acids at positions 232 to 246 of the fibrinogen γ chain are bound to each other in a fibrinogen molecule. Further, it is shown that when fibrinogen is converted to insoluble fibrin, the binding is released, and these sites are exposed. It is also found that antibodies obtained by immunization with these sites do not bind to fibrinogen and have high affinity for and high specificity to insoluble fibrin.

摘要翻译： 为了提供不与纤维蛋白原结合并且对不溶性纤维蛋白具有高亲和力和高特异性的抗体，发现包含纤维蛋白原Bβ链的第231至246位的氨基酸的位点和 包含纤维蛋白原γ链的第232至246位的氨基酸的位点在纤维蛋白原分子中彼此结合。 此外，显示当纤维蛋白原转化为不溶性纤维蛋白时，释放结合，并暴露这些部位。 还发现通过用这些位点进行免疫获得的抗体不结合纤维蛋白原并且对不溶性纤维蛋白具有高亲和力和高特异性。