公开(公告)号：US12066429B2

公开(公告)日：2024-08-20

申请号：US16913376

申请日：2020-06-26

申请人： National University Corporation Tokyo Medical and Dental University ,  Nippon Zoki Pharmaceutical Co., Ltd.

发明人： Hiroshi Asahara ,  Tomoki Chiba ,  Kentaro Abe

IPC分类号： C12N15/11 ,  C12N15/10 ,  C12N15/113 ,  C12N15/63 ,  G01N33/50

CPC分类号： G01N33/5023 ,  C12N15/1086 ,  C12N15/113 ,  C12N15/63 ,  C12N2310/14 ,  C12N2310/18 ,  C12N2320/10 ,  C12N2320/50 ,  G01N2500/02 ,  G01N2500/10

摘要： Provided are an expression inhibitor of an inflammation promoting factor based on the discovery of a new factor influencing the expression amount/level of an inflammation promoting factor, and a development tool therefor. Provided are also a diagnostic agent and a diagnosis method for immune diseases, inflammatory diseases, painful conditions and similar. More specifically provided are: an expression inhibitor of an inflammation promoting factor containing at least one kind of inhibitor selected from the group consisting of RBMS2 expression inhibitor and RBMS2 function inhibitor; a screening method using as an indicator the expression or the function of RBMS2; an expression cassette useful for the method; as well as a diagnostic agent containing a product detection agent for RBMS2 gene expression and disease detection method using as an indicator RBMS2 gene expression amount/level.

公开(公告)号：US11773376B2

公开(公告)日：2023-10-03

申请号：US16630188

申请日：2018-07-12

申请人： National University Corporation Tokyo Medical and Dental University

发明人： Hiroshi Asahara ,  Kensuke Kataoka ,  Tomoki Chiba

IPC分类号： C12N5/077 ,  A61L27/22 ,  A61L27/36 ,  A61L27/38

CPC分类号： C12N5/066 ,  A61L27/225 ,  A61L27/3683 ,  A61L27/386 ,  A61L27/3834 ,  A61L2430/10 ,  C12N2506/45 ,  C12N2527/00 ,  C12N2533/54 ,  C12N2533/56

摘要： The present invention relates to providing artificial tendon or ligament tissue having sufficient strength. More specifically, artificial tendon or ligament tissue having sufficient strength is provided by embedding collagen-secreting cells in a gel having strength capable of resisting a tensile load and by culturing the cells while applying a tensile load to the gel to produce artificial tendon or ligament tissue. Cells that steadily express the Mkx gene can be used as the collagen-secreting cells. A fibrin gel containing aprotinin can be used as the gel.