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公开(公告)号:US20140370038A1
公开(公告)日:2014-12-18
申请号:US14312117
申请日:2014-06-23
IPC分类号: C12N5/0783 , A61K38/20 , A61K38/21 , A61K35/14
CPC分类号: C12N5/0637 , A61K35/17 , A61K38/1793 , A61K38/20 , A61K38/2033 , A61K38/208 , A61K38/217 , A61K39/001 , A61K2039/5158 , C07K16/244 , C07K2317/76 , C12N2501/2302 , C12N2501/2304 , C12N2501/2305 , C12N2501/2312 , C12N2501/2323 , C12N2501/24 , G01N33/505 , G01N33/5094 , G01N2333/52 , G01N2333/54 , G01N2333/5406 , G01N2333/5409 , G01N2333/5434 , G01N2333/55 , G01N2333/57 , G01N2333/70514 , G01N2333/70596 , G01N2333/715 , G01N2800/245 , A61K2300/00
摘要: The invention relates to a method of assessing whether a subject includes CD4+,CD25+ T cells that have been activated to a specific antigen. The method includes the steps of obtaining from the subject a sample of lymphocytes including CD4+,CD25+ T cells, incubating at least one portion of the sample of lymphocytes so as to promote, distinction of CD4+,CD25+ T cells that have been activated to the specific antigen from those CD4+,CD25+ T cells that have not been activated to the specific antigen, and thereafter determining whether CD4+,CD25+ T cells activated to the specific antigen are present in the sample. The invention further relates to methods of growing CD4+, CD25+ T cells that have been activated to a specific antigen in vitro and to methods of increasing tolerance in a subject using the CD4+, CD25+ T cells that have been grown in vitro.
摘要翻译: 本发明涉及一种评估受试者是否包括已被激活到特定抗原的CD4 +,CD25 + T细胞的方法。 该方法包括以下步骤:从受试者获得包括CD4 +,CD25 + T细胞的淋巴细胞样品,培养淋巴细胞样品的至少一部分,以促进已被激活的CD4 +,CD25 + T细胞与特异性 来自没有被激活到特定抗原的那些CD4 +,CD25 + T细胞的抗原,然后确定在特定抗原中活化的CD4 +,CD25 + T细胞是否存在于样品中。 本发明还涉及在体外培养已被激活到特定抗原的CD4 +,CD25 + T细胞的方法和使用已经在体外培养的CD4 +,CD25 + T细胞增加受试者耐受性的方法。