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18 条记录 (耗时 0.043 秒)

    Electrochemical detection of mismatch nucleic acids
    3.
    发明授权
    Electrochemical detection of mismatch nucleic acids 有权
    错配核酸的电化学检测

    公开(公告)号：US07291457B2

    公开(公告)日：2007-11-06

    申请号：US10177537

    申请日：2002-06-21

    申请人： Jeffrey F. Miller ,  Jun Huang ,  Tza-Huei Wang ,  Chih-Ming Ho ,  Minghsun Liu

    发明人： Jeffrey F. Miller ,  Jun Huang ,  Tza-Huei Wang ,  Chih-Ming Ho ,  Minghsun Liu

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q2565/607 ,  C12Q2537/125 ,  C12Q2525/301 ,  C12Q2563/113

    摘要： A method and system for specifically detecting perfectly matched probe-target hybrids by annealing a hairpin-based probe to a nucleic acid target under conditions favoring the formation of perfectly matched targets over mismatched targets, and electrochemically detecting the annealed probe. Sensitivity of detection can be enhanced by catalyzed reporter deposition of probe signal prior to electrochemical detection. Specificity can be enhanced by including hairpin-based competitor probes in the annealing step. The detection assay is particularly applicable to typing single nucleotide polymorphisms by DNA sandwich hybridization.

    摘要翻译： 一种用于特异性检测完全匹配的探针 - 靶杂交体的方法和系统，所述方法和系统通过在有助于在失配靶上形成完全匹配的靶的条件下将发夹基探针退火至核酸靶，以及电化学检测退火的探针。 在电化学检测之前，探测信号的催化报道沉积可以提高检测灵敏度。 通过在退火步骤中包含基于发夹的竞争者探针可以提高特异性。 检测方法特别适用于通过DNA夹心杂交分型单核苷酸多态性。

    System, process, or method for the use of cross-inhibitive-voting in collaborative societal decision making within social networks
    10.
    发明申请
    System, process, or method for the use of cross-inhibitive-voting in collaborative societal decision making within social networks 审中-公开
    在社交网络中协同社会决策中使用交叉抑制投票的制度，过程或方法

    公开(公告)号：US20150254918A1

    公开(公告)日：2015-09-10

    申请号：US14201672

    申请日：2014-03-07

    申请人： Jeffrey F. Miller

    发明人： Jeffrey F. Miller

    IPC分类号： G07C13/00

    CPC分类号： G07C13/00 ,  G06Q2230/00

    摘要： As uses of interne based social networks have become broader, the potential for collaborative interaction has remained restricted by the effectiveness of voting methods. The application of cross-inhibitive-voting in social networks enables improved quality in the outcome of voting processes, and inclusion of greater numbers of voters by employing repetitive voting, quorum detection, reciprocal-time-weighting of votes, and the determination of a single best-of-N, or top-x-of-N ranking of alternatives determined by the number of in-favor votes minus the number of in-opposition votes.

    摘要翻译： 随着基于互联网的社交网络的使用越来越广泛，协作互动的潜力仍然受到投票方法的有效性的限制。 交叉抑制投票在社交网络中的应用可以提高投票过程的质量，并通过采用重复投票，法定人数检测，互动时间权重以及确定单个投票来包含更多选民。 最佳N或最高排名的替代品排名由赞成票数减去反对票数量决定。