公开(公告)号：US06923993B2

公开(公告)日：2005-08-02

申请号：US10020816

申请日：2001-12-12

申请人： Nicholas D. Donato ,  Nicholas J. Donato ,  David C. Sample ,  Margot Perez ,  John S. McMurray ,  Robert A Newman

发明人： Nicholas D. Donato ,  Nicholas J. Donato ,  David C. Sample ,  Margot Perez ,  John S. McMurray ,  Robert A Newman

IPC分类号： A61K36/47 ,  A61P35/00 ,  A61P43/00 ,  C12N20060101 ,  C12N5/22 ,  A61K35/78

CPC分类号： A61K36/47

摘要： The present invention is directed to a process of isolating an extract from a Euphorbia obesa (EO) plant by: preparing a sample of said plant comprising removal of the latex material; dissolving said sample with first solvent to form a solution; separating said solution into a liquid and a pulp fraction; and purifying said pulp fraction. The isolated EO extract induces apoptosis and inhibits growth of a cancerous cell. Thus, the present invention is also directed to a method for inducing apoptosis and growth inhibition of a cancerous cell by contacting the cell with an effective amount of the EO extract by the process of the invention. Preferably, the extract is administered both to the tumor directly and intravenously. The preferred lines of cancerous cells are melanoma, non-small cell lung cancer, prostate cancer, breast carcinoma, ovarian cancer, lymphoma and leukemia cells.

摘要翻译： 本发明涉及通过以下步骤从大戟属植物（Euphorbia obesa，EO）植物中分离提取物的方法：制备所述植物的样品，包括除去胶乳材料; 用第一溶剂溶解所述样品以形成溶液; 将所述溶液分离成液体和纸浆馏分; 并纯化所述纸浆级分。 分离的EO提取物诱导凋亡并抑制癌细胞的生长。 因此，本发明还涉及通过本发明的方法使细胞与有效量的EO提取物接触来诱导癌细胞的凋亡和生长抑制的方法。 优选地，将提取物直接和静脉内施用于肿瘤。 癌细胞的优选系是黑素瘤，非小细胞肺癌，前列腺癌，乳腺癌，卵巢癌，淋巴瘤和白血病细胞。