公开(公告)号：US20130309753A1

公开(公告)日：2013-11-21

申请号：US13473566

申请日：2012-05-16

申请人： Nicola Pozzi ,  Enrico Di Cera ,  Sergio Barranco-Medina

发明人： Nicola Pozzi ,  Enrico Di Cera ,  Sergio Barranco-Medina

IPC分类号： C12N9/50

CPC分类号： C12N9/6429 ,  C12N9/6408 ,  C12Y304/21005

摘要： A recombinant serine protease precursor that auto-activates in an aqueous buffer to form a mature active enzyme is disclosed. A contemplated precursor contains 1 to about 10 heterologous amino acid residues that function to enhance by at least ten-fold the room temperature rate of auto-lytic bond cleavage to form the active enzyme relative to the auto-lytic cleavage rate of the native enzyme precursor when each precursor is dispersed in an aqueous buffer at an optimal pH value for the proteolytic activity of the protease. Illustrative active enzymes include serine proteases such as thrombin and protein C. A method of preparing and using an enzyme precursor is also disclosed.

摘要翻译： 公开了一种在水性缓冲液中自动激活以形成成熟活性酶的重组丝氨酸蛋白酶前体。 预期的前体含有1至约10个异源氨基酸残基，其功能相对于天然酶前体的自溶裂解速率增强自溶裂键切割的室温速率至少10倍以形成活性酶 当每种前体分散在蛋白酶的蛋白水解活性的最佳pH值的水性缓冲液中时。 说明性的活性酶包括丝氨酸蛋白酶如凝血酶和蛋白C.还公开了制备和使用酶前体的方法。