公开(公告)号：US08852593B2

公开(公告)日：2014-10-07

申请号：US13515650

申请日：2010-12-17

申请人： Nicolas Baurin ,  Francis Blanche ,  Beatrice Cameron ,  Carsten Corvey ,  Tarik Dabdoubi ,  Christian Engel ,  Peter Florian ,  Ingo Focken ,  Katja Kroll ,  Jochen Kruip ,  Christian Lange ,  Thomas Langer ,  Martin Lorenz ,  Vincent Mikol ,  Ercole Rao ,  Peter Wonerow

发明人： Nicolas Baurin ,  Francis Blanche ,  Beatrice Cameron ,  Carsten Corvey ,  Tarik Dabdoubi ,  Christian Engel ,  Peter Florian ,  Ingo Focken ,  Katja Kroll ,  Jochen Kruip ,  Christian Lange ,  Thomas Langer ,  Martin Lorenz ,  Vincent Mikol ,  Ercole Rao ,  Peter Wonerow

IPC分类号： A61K39/395 ,  C07K16/28 ,  A61K39/00

CPC分类号： C07K16/28 ,  A61K2039/505 ,  C07K1/1075 ,  C07K16/2803 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/41 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92

摘要： The present invention discloses novel antibodies that specifically bind to the human platelet membrane protein Glycoprotein VI (GPVI) and their monovalent fragments or derivatives. The antibodies of the invention are antibodies from hybridoma clone 390 and fragment antibodies thereof able to induce a GPVI depletion phenotype. These antibodies and Fab fragments are able to block collagen binding and thus preventing platelet activation by collagen. The invention also relates to hybridoma clones and expression plasmids for the production of disclosed antibodies and Fab fragments. The present invention further refers to the uses of monovalent antibody fragments to manufacture research, diagnostic and immunotherapeutic agents for the treatment of thrombosis and other vascular diseases. The invention also concerns a Fab bearing a molecule at the C-terminal extremity, as well as method for prevention of recognition of Fab by antibodies using such modified Fab. The invention concerns a method for prevention of platelet activation when an anti-GP VI Fab is used.

摘要翻译： 本发明公开了特异性结合人血小板膜蛋白Glycoprotein VI（GPVI）及其单价片段或衍生物的新型抗体。 本发明的抗体是来自杂交瘤克隆390的抗体及其能诱导GPVI消耗表型的片段抗体。 这些抗体和Fab片段能够阻断胶原结合，从而阻止胶原的血小板活化。 本发明还涉及用于产生所公开的抗体和Fab片段的杂交瘤克隆和表达质粒。 本发明还涉及一价抗体片段用于制备用于治疗血栓形成和其它血管疾病的研究，诊断和免疫治疗剂的用途。 本发明还涉及在C-末端具有分子的Fab，以及使用这种修饰的Fab预防用抗体识别Fab的方法。 本发明涉及当使用抗GP VI Fab时预防血小板活化的方法。

公开(公告)号：US20120244152A1

公开(公告)日：2012-09-27

申请号：US13515650

申请日：2010-12-17

申请人： Nicolas Baurin ,  Francis Blanche ,  Beatrice Cameron ,  Carsten Corvey ,  Tarik Dabdoubi ,  Christian Engel ,  Peter Florian ,  Ingo Focken ,  Katja Kroll ,  Jochen Kruip ,  Christian Lange ,  Thomas Langer ,  Martin Lorenz ,  Vincent Mikol ,  Ercole Rao ,  Peter Wonerow

发明人： Nicolas Baurin ,  Francis Blanche ,  Beatrice Cameron ,  Carsten Corvey ,  Tarik Dabdoubi ,  Christian Engel ,  Peter Florian ,  Ingo Focken ,  Katja Kroll ,  Jochen Kruip ,  Christian Lange ,  Thomas Langer ,  Martin Lorenz ,  Vincent Mikol ,  Ercole Rao ,  Peter Wonerow

IPC分类号： A61K39/395 ,  C07K16/28 ,  A61P9/00 ,  C12P21/02 ,  A61P7/02 ,  C07K1/00 ,  C12N15/13

CPC分类号： C07K16/28 ,  A61K2039/505 ,  C07K1/1075 ,  C07K16/2803 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/41 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92

摘要： The present invention discloses novel antibodies that specifically bind to the human platelet membrane protein Glycoprotein VI (GPVI) and their monovalent fragments or derivatives. The antibodies of the invention are antibodies from hybridoma clone 390 and fragment antibodies thereof able to induce a GPVI depletion pheno-type. These antibodies and Fab fragments are able to block collagen binding and thus preventing platelet activation by collagen. The invention also relates to hybridoma clones and expression plasmids for the production of disclosed antibodies and Fab fragments. The present invention further refers to the uses of monovalent antibody fragments to manufacture research, diagnostic and immunotherapeutic agents for the treatment of thrombosis and other vascular diseases. The invention also concerns a Fab bearing a molecule at the C-terminal extremity, as well as method for prevention of recognition of Fab by antibodies using such modified Fab. The invention concerns a method for prevention of platelet activation when an anti-GP VI Fab is used.

摘要翻译： 本发明公开了特异性结合人血小板膜蛋白Glycoprotein VI（GPVI）及其一价片段或衍生物的新型抗体。 本发明的抗体是来自杂交瘤克隆390的抗体及其能诱导GPVI消耗物质型的片段抗体。 这些抗体和Fab片段能够阻断胶原结合，从而阻止胶原的血小板活化。 本发明还涉及用于产生所公开的抗体和Fab片段的杂交瘤克隆和表达质粒。 本发明还涉及一价抗体片段用于制备用于治疗血栓形成和其它血管疾病的研究，诊断和免疫治疗剂的用途。 本发明还涉及在C-末端具有分子的Fab，以及使用这种修饰的Fab预防用抗体识别Fab的方法。 本发明涉及当使用抗GP VI Fab时预防血小板活化的方法。