公开(公告)号：US20140072954A1

公开(公告)日：2014-03-13

申请号：US14110980

申请日：2012-04-02

申请人： Nobuyoshi Umeda ,  Nobuhiko Sato ,  Ayako Tsukamoto

发明人： Nobuyoshi Umeda ,  Nobuhiko Sato ,  Ayako Tsukamoto

IPC分类号： C12N5/0786

CPC分类号： C12N5/0645 ,  C12N5/0087 ,  G01N33/491

摘要： The present invention aims to provide a mononuclear cell preparation material capable of improving the mononuclear cell recovery while reducing granulocyte contamination, and a mononuclear cell preparation method which improves the mononuclear cell recovery. Provided is a mononuclear cell preparation material for removing granulocytes from blood to prepare mononuclear cells, which includes a nonwoven fabric made of a polyamide resin. Also provided is a method for preparing mononuclear cells using a device with the mononuclear cell preparation material packed in a container, the method including the steps of: (A) passing blood through the mononuclear cell preparation device from an inlet side to an outlet side of the device, thereby capturing granulocytes in the device; and (B) passing a washing solution through the mononuclear cell preparation device from the inlet side to the outlet side of the device, thereby recovering mononuclear cells remaining in the device.

摘要翻译： 本发明旨在提供能够改善单核细胞恢复同时减少粒细胞污染的单核细胞制备材料，以及改善单核细胞恢复的单核细胞制备方法。 提供一种用于从血液中除去粒细胞以制备单核细胞的单核细胞制备材料，其包括由聚酰胺树脂制成的无纺布。 还提供了使用包装在容器中的单核细胞制备材料的装置制备单核细胞的方法，所述方法包括以下步骤：（A）将血液通过单核细胞制备装置从入口侧到出口侧 从而在装置中捕获粒细胞; 和（B）将清洗液从装置的入口侧通过单核细胞制备装置，从而回收剩余在装置中的单核细胞。

公开(公告)号：US20130323712A1

公开(公告)日：2013-12-05

申请号：US13989523

申请日：2010-11-25

申请人： Nobuhiko Sato ,  Ayako Tsukamoto

发明人： Nobuhiko Sato ,  Ayako Tsukamoto

IPC分类号： C12N5/078

CPC分类号： C12N5/0634 ,  A61K35/14 ,  C12M47/04 ,  C12N1/04

摘要： An object of the present invention is to provide a separation system and a separation material for efficiently separating white blood cells or mononuclear cells from a biological fluid containing blood cell components. White blood cells or mononuclear cells can be efficiently separated by contacting a biological fluid containing blood cell components with a separation material having an average fiber diameter of at least 2.0 μm but not more than 6.0 μm and an air permeability coefficient M of at least 6.2 but not more than 35 to capture white blood cells on the separation material, and recovering the captured white blood cells or mononuclear cells using a detachment solution.

摘要翻译： 本发明的目的是提供一种用于从含有血细胞成分的生物流体中有效分离白细胞或单核细胞的分离系统和分离材料。 通过使含有血液细胞成分的生物液体与平均纤维直径为至少2.0μm，但不超过6.0μm，透气系数M为6.2以上的分离材料接触，能够有效地分离白细胞或单核细胞 不超过35个以捕获分离材料上的白细胞，并使用分离溶液回收捕获的白细胞或单核细胞。