公开(公告)号：US08137963B2

公开(公告)日：2012-03-20

申请号：US12438545

申请日：2007-08-20

申请人： Noriaki Shimizu ,  Toshihiko Hashizume ,  Masashi Shimizu

发明人： Noriaki Shimizu ,  Toshihiko Hashizume ,  Masashi Shimizu

IPC分类号： C12N15/63 ,  C12N15/85 ,  C12P19/34

CPC分类号： C12N15/67 ,  C12N2510/02 ,  C12N2800/108 ,  C12N2820/85 ,  C12N2830/46

摘要： A vector of the present invention is a vector for amplifying a target gene in a mammalian cell, the vector including an amplification-activating fragment, which is a partial fragment of a mammalian replication initiation region and has a gene amplification activity site, and a mammalian nuclear matrix attachment region. In the case where the mammalian replication initiation region as described above derives from a c-myc locus, for example, the above-described partial fragment at least contains a duplex unwinding element and a topoisomerase II-binding domain. The vector as described above improves gene transfer efficiency and gene amplification efficiency compared with the existing high gene amplification systems. Thus, a method whereby a “high gene amplification system” developed by the inventors can amplify a target gene with better gene transfer efficiency and a vector to be used in this method are provided.

摘要翻译： 本发明的载体是用于扩增哺乳动物细胞中的靶基因的载体，所述载体包含作为哺乳动物复制起始区域的部分片段的扩增活化片段，并具有基因扩增活性位点和哺乳动物 核基质附着区。 在如上所述的哺乳动物复制起始区源自c-myc基因座的情况下，例如，上述部分片段至少含有双链体展开元件和拓扑异构酶II结合结构域。 如上所述的载体与现有的高基因扩增系统相比提高了基因转移效率和基因扩增效率。 因此，提供了本发明人开发的“高基因扩增系统”可以扩增具有更好的基因转移效率的靶基因和用于该方法的载体的方法。

公开(公告)号：US20100317060A1

公开(公告)日：2010-12-16

申请号：US12438545

申请日：2007-08-20

申请人： Noriaki Shimizu ,  Toshihiko Hashizume ,  Masashi Tsuruga-shi

发明人： Noriaki Shimizu ,  Toshihiko Hashizume ,  Masashi Tsuruga-shi

IPC分类号： C12P19/34 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N15/67 ,  C12N2510/02 ,  C12N2800/108 ,  C12N2820/85 ,  C12N2830/46

摘要： A vector of the present invention is a vector for amplifying a target gene in a mammalian cell, the vector including an amplification-activating fragment, which is a partial fragment of a mammalian replication initiation region and has a gene amplification activity site, and a mammalian nuclear matrix attachment region. In the case where the mammalian replication initiation region as described above derives from a c-myc locus, for example, the above-described partial fragment at least contains a duplex unwinding element and a topoisomerase II-binding domain. The vector as described above improves gene transfer efficiency and gene amplification efficiency compared with the existing high gene amplification systems. Thus, a method whereby a “high gene amplification system” developed by the inventors can amplify a target gene with better gene transfer efficiency and a vector to be used in this method are provided.

摘要翻译： 本发明的载体是用于扩增哺乳动物细胞中的靶基因的载体，该载体包含作为哺乳动物复制起始区域的部分片段的扩增活化片段，并具有基因扩增活性位点和哺乳动物 核基质附着区。 在如上所述的哺乳动物复制起始区源自c-myc基因座的情况下，例如，上述部分片段至少含有双链体展开元件和拓扑异构酶II结合结构域。 如上所述的载体与现有的高基因扩增系统相比提高了基因转移效率和基因扩增效率。 因此，提供了本发明人开发的“高基因扩增系统”可以扩增具有更好的基因转移效率的靶基因和用于该方法的载体的方法。