公开(公告)号：US09018444B2

公开(公告)日：2015-04-28

申请号：US13057818

申请日：2009-08-07

申请人： Noriho Fukuzawa ,  Takeshi Matsumura ,  Noriko Itchoda ,  Takeaki Ishihara ,  Chikara Masuta

发明人： Noriho Fukuzawa ,  Takeshi Matsumura ,  Noriko Itchoda ,  Takeaki Ishihara ,  Chikara Masuta

IPC分类号： C12N15/83 ,  C12N15/33 ,  C12N15/82 ,  C12N15/86

CPC分类号： C12N15/8203 ,  C12N15/86 ,  C12N2770/14043

摘要： The present invention is to provide a novel non-diffusing plant virus vector wherein virus vector infection and proliferation are possible only in a recombinant plant transformed with a gene necessary for viral proliferation, thereby enabling avoidance of unintended diffusion of a recombinant virus, a selective and specific expression system therefor, and a method of expression thereof which comprises combining a non-diffusing virus vector lacking a gene involved in intercellular movement of a cucumber mosaic virus (CMV) genome and a transgenic plant transformed with the lacked gene involved in intercellular movement for the non-diffusing virus vector to establish infection and proliferation selectively and specifically in the transgenic plant.

摘要翻译： 本发明提供一种新的非扩散性植物病毒载体，其中病毒载体的感染和增殖仅在用病毒增殖所必需的基因转化的重组植物中是可能的，从而能够避免重组病毒的非预期扩散，选择性和 特异性表达系统及其表达方法，其包括将缺乏参与黄瓜花叶病毒（CMV）基因组的细胞间运动的基因的非扩散病毒载体与转基因植物相结合，转基因植物参与细胞间移动 非扩散病毒载体在转基因植物中选择性和特异性地建立感染和增殖。

公开(公告)号：US20110138497A1

公开(公告)日：2011-06-09

申请号：US13057818

申请日：2009-08-07

申请人： Noriho Fukuzawa ,  Takeshi Matsumura ,  Noriko Itchoda ,  Takeaki Ishihara ,  Chikara Masuta

发明人： Noriho Fukuzawa ,  Takeshi Matsumura ,  Noriko Itchoda ,  Takeaki Ishihara ,  Chikara Masuta

IPC分类号： A01H1/06 ,  C12N15/63 ,  A01H5/00

CPC分类号： C12N15/8203 ,  C12N15/86 ,  C12N2770/14043

摘要： The present invention is to provide a novel non-diffusing plant virus vector wherein virus vector infection and proliferation are possible only in a recombinant plant transfected with a gene necessary for viral proliferation, thereby enabling avoidance of unintended diffusion of a recombinant virus, a selective and specific expression system therefor, and a method of expression thereof which comprises combining a non-diffusing virus vector lacking a gene involved in intercellular movement of a cucumber mosaic virus (CMV) genome and a transgenic plant transfected with the lacked gene involved in intercellular movement for the non-diffusing virus vector to establish infection and proliferation selectively and specifically in the transgenic plant.

摘要翻译： 本发明提供一种新的非扩散性植物病毒载体，其中病毒载体的感染和增殖仅在用病毒增殖所必需的基因转染的重组植物中是可能的，从而能够避免重组病毒的非预期扩散，选择性和 特异性表达系统及其表达方法，其包括将缺少涉及黄瓜花叶病毒（CMV）基因组的细胞间运动的基因的非扩散病毒载体与转染有参与细胞间移动的缺失基因的转基因植物组合 非扩散病毒载体在转基因植物中选择性和特异性地建立感染和增殖。