公开(公告)号：US20200248157A1

公开(公告)日：2020-08-06

申请号：US16837166

申请日：2020-04-01

Applicant: Novozymes A/S

Inventor： Tomoko Matsui ,  Akihiko YAMAGISHI

IPC: C12N9/44 ,  C12P7/06 ,  C12P19/02 ,  C12P19/16

Abstract: The present invention relates to pullulanase variants comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20180298364A1

公开(公告)日：2018-10-18

申请号：US16016950

申请日：2018-06-25

Applicant: NOVOZYMES A/S

Inventor： Tomoko MATSUI ,  Akihiko YAMAGISHI

IPC: C12N9/44 ,  C12P7/06 ,  C12P19/16 ,  C12P19/02

Abstract: The present invention relates to pullulanase variants comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20170044511A1

公开(公告)日：2017-02-16

申请号：US15112096

申请日：2015-01-21

Applicant: Novozymes A/S

Inventor： Tomoko MATSUI ,  Akihiko YAMAGISHI

IPC: C12N9/44 ,  C12P19/02 ,  C12P19/16

CPC classification number: C12N9/2457 ,  C12P7/06 ,  C12P19/02 ,  C12P19/16 ,  C12P2203/00 ,  C12Y302/01041 ,  Y02E50/17

Abstract: The present invention relates to pullulanase variants having increased thermoactivity and comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The variants are mainly derived from pullulanases from Bacillus deramificans, Bacillus acidopullulyticus or hybrid pullulanases.

Abstract translation: 本发明涉及具有增加的热活性的支链淀粉酶变体，并且包括在对应于位置393,143,150,243,244,345,346,368,370,373,381,368,385,337,381,382,385的一个或多个位置上的母体支链淀粉酶的取代 ，387,402,49,430,431,432,456,468,492,610,624,626,632,665和699的多核苷酸。本发明还涉及编码变体的多核苷酸 ; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。 变异体主要衍生自来自芽孢杆菌属（Bacillus deramificans），Bacillus acidulullulyticus或杂合支链淀粉酶的支链淀粉酶。