公开(公告)号：US20210301285A1

公开(公告)日：2021-09-30

申请号：US17265092

申请日：2019-07-19

Applicant: Novozymes A/S

Inventor： Michael Lynge Nielsen ,  Nicholas Jochumsen ,  José Arnau

IPC: C12N15/10 ,  C12N15/81 ,  C12N15/90

Abstract: Means and methods for preparing combinatorial libraries of DNA constructs, in particular expression cassettes, including nucleic acid constructs, expression vectors, host cells, methods for preparing host cells, and methods for producing polypeptides of interest, whereby the expression comprises an first intron and a second intron on either side of the polynucleotide to be expressed and a promoter and a terminator. Also claimed is a method of constructing eukaryotic host cells in which the cells are contacted with three polynucleotides and in which the first and second and the second and third are pairwise capable of homologous recombination and of subsequent formation of introns.