公开(公告)号：US20220340843A1

公开(公告)日：2022-10-27

申请号：US17763592

申请日：2020-10-01

Applicant: Novozymes A/S

Inventor： Lone Baunsgaard ,  Michael Lynge Nielsen ,  Kenneth Jensen ,  Hiroshi Teramoto ,  Keiichi Ayabe

IPC: C11D3/386 ,  C12N9/42 ,  C11D11/00

Abstract: Disclosed are fusion polypeptides comprising at least two carbohydrate binding modules (CBMs), wherein the polypeptide has carbohydrate binding activity. Also disclosed is the use of such polypeptides for reducing the wrinkles in laundry, as well as detergent compositions and laundry booster compositions comprising the same. Also disclosed are polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the variants and methods of making the variants.

公开(公告)号：US11091753B2

公开(公告)日：2021-08-17

申请号：US16617430

申请日：2018-05-31

Applicant: Novozymes A/S

Inventor： Michael Lynge Nielsen ,  Nicholas Jochumsen

IPC: C12N9/90 ,  C12P7/06 ,  C12P7/10 ,  C12N1/20 ,  C12N1/14 ,  C12N9/92 ,  C12N1/16 ,  C12N15/81

Abstract: Provided herein are recombinant host cells having a heterologous polynucleotide encoding a xylose isomerase, wherein the cells are capable of improved growth on xylose. Also described are processes of fermenting saccharified material using the recombinant cells to produce ethanol at higher yields.

公开(公告)号：US20200172937A1

公开(公告)日：2020-06-04

申请号：US16617430

申请日：2018-05-31

Applicant: Novozymes A/S

Inventor： Michael Lynge Nielsen ,  Nicholas Jochumsen

IPC: C12P7/10 ,  C12N9/92 ,  C12N15/81 ,  C12N1/16

Abstract: Provided herein are recombinant host cells having a heterologous polynucleotide encoding a xylose isomerase, wherein the cells are capable of improved growth on xylose. Also described are processes of fermenting saccharified material using the recombinant cells to produce ethanol at higher yields.

公开(公告)号：US20210301285A1

公开(公告)日：2021-09-30

申请号：US17265092

申请日：2019-07-19

Applicant: Novozymes A/S

Inventor： Michael Lynge Nielsen ,  Nicholas Jochumsen ,  José Arnau

IPC: C12N15/10 ,  C12N15/81 ,  C12N15/90

Abstract: Means and methods for preparing combinatorial libraries of DNA constructs, in particular expression cassettes, including nucleic acid constructs, expression vectors, host cells, methods for preparing host cells, and methods for producing polypeptides of interest, whereby the expression comprises an first intron and a second intron on either side of the polynucleotide to be expressed and a promoter and a terminator. Also claimed is a method of constructing eukaryotic host cells in which the cells are contacted with three polynucleotides and in which the first and second and the second and third are pairwise capable of homologous recombination and of subsequent formation of introns.

公开(公告)号：US20160244737A1

公开(公告)日：2016-08-25

申请号：US14913967

申请日：2014-08-22

Applicant: Novozymes A/S

Inventor： Hiroshi Teramoto ,  Hiroaki Udagawa ,  Jan Lehmbeck ,  Michael Lynge Nielsen

IPC: C12N9/62 ,  C12N9/18 ,  C12N15/80 ,  C12N9/20 ,  C12P21/02 ,  C12N9/82

CPC classification number: C12N9/62 ,  C07K14/38 ,  C12N9/0036 ,  C12N9/18 ,  C12N9/20 ,  C12N9/58 ,  C12N9/82 ,  C12N15/80 ,  C12P21/02

Abstract: The present invention relates to a recombinant fungal host cell comprising at least one first polynucleotide encoding a polypeptide of interest; and one or more second polynucleotide encoding a fungal PepC protease, wherein the one or more second polynucleotide is operably linked to a regulated heterologous promoter, as well as a method for producing a polypeptide of interest, comprising cultivating said fungal host cell.

Abstract translation: 本发明涉及重组真菌宿主细胞，其包含至少一种编码目的多肽的第一多核苷酸; 和编码真菌PepC蛋白酶的一种或多种第二多核苷酸，其中所述一种或多种第二多核苷酸可操作地连接到受调节的异源启动子，以及用于产生感兴趣多肽的方法，包括培养所述真菌宿主细胞。