公开(公告)号：US20190169238A1

公开(公告)日：2019-06-06

申请号：US16319780

申请日：2017-07-19

Applicant: Novozymes A/S

Inventor： Hiroshi Teramoto ,  Kaihei Kojima ,  Preethi Ramaiya

IPC: C07K14/38 ,  C12N15/80 ,  C12P21/02

Abstract: The instant invention relates to filamentous fungal host cells producing and secreting a heterologous polypeptide of interest, said mutant host cell comprising and expressing a mutated ireA gene or a homologue thereof encoding a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in 5 positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16, as well as methods of producing a polypeptide of interest in said cells and methods for constructing said cells.

公开(公告)号：US11046736B2

公开(公告)日：2021-06-29

申请号：US16319780

申请日：2017-07-19

Applicant: Novozymes A/S

Inventor： Hiroshi Teramoto ,  Kaihei Kojima ,  Preethi Ramaiya

IPC: C12N1/14 ,  C12N15/80 ,  C07K14/38 ,  C12P21/02

Abstract: The instant invention relates to filamentous fungal host cells producing and secreting a heterologous polypeptide of interest, said mutant host cell comprising and expressing a mutated ireA gene or a homologue thereof encoding a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in 5 positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16, as well as methods of producing a polypeptide of interest in said cells and methods for constructing said cells.