公开(公告)号：US20140335594A1

公开(公告)日：2014-11-13

申请号：US14364928

申请日：2012-12-19

Applicant: Novozymes A/S

Inventor： Poul Johansen ,  Cecilia Jansson Kepka

IPC: C07K1/30

CPC classification number: C07K1/30 ,  A61L2/0011

Abstract: The present invention relates to a method for selectively removing virus and/or virus DNA from a solution comprising a target protein, whereby acetone is used as a precipitation agent to precipitate virus and/or virus DNA as well as the target protein.

Abstract translation: 本发明涉及一种从包含靶蛋白的溶液中选择性除去病毒和/或病毒DNA的方法，其中使用丙酮作为沉淀剂沉淀病毒和/或病毒DNA以及靶蛋白。

公开(公告)号：US09676817B2

公开(公告)日：2017-06-13

申请号：US14364928

申请日：2012-12-19

Applicant: Novozymes A/S

Inventor： Poul Johansen ,  Cecilia Jansson Kepka

IPC: C07K1/36 ,  C07K1/30 ,  A61L2/00 ,  C07K1/00

CPC classification number: C07K1/30 ,  A61L2/0011

Abstract: The author attempted and succeeded in separating and purifying the cysteine protease inhibitors from new gingers. The root and stem of new gingers were extracted with neutral buffer which subsequently underwent heat treatment at 80° C. for 10 minutes. After centrifugal separation, the supernatant was separated with acetone. The fractions that precipitated at an acetone concentration of 55-77% were injected into a chromatographic column of DEAE-cellulose and Sephadex G-75 gel for purification. The results demonstrated that there were two cysteine protease inhibitors with a molecular weight of 11,000-11,800 and 15,500-16,000, respectively, which strongly inhibit papain. The two cysteine protease inhibitors do not have high heat stability.