公开(公告)号：US20150031621A1

公开(公告)日：2015-01-29

申请号：US14327110

申请日：2014-07-09

申请人： OCTAPHARMA AG

发明人： Hubert Brandstaetter ,  Petra Schulz ,  Juergen Roemisch

IPC分类号： C07K14/47

CPC分类号： C07K14/472 ,  A61K38/1709 ,  C07K1/36 ,  C07K14/435 ,  C07K14/47

摘要： A method for purification of complement Factor H from a complement Factor H containing source such as blood or blood plasma, in particular a caprylate precipitate of a Factor H containing source, which is e.g. obtained by addition of caprylate ions to fractions of blood or plasma, comprising the steps of: a) providing a Factor H containing source, in particular reconstitution of caprylate precipitate to provide a complement Factor H containing solution; b) performing a cation exchange chromatography in particular as first chromatographic step; c) performing an anion exchange chromatography; d) performing a hydroxyl apatite chromatography; e) followed by ultra/diafiltration to obtain a complement Factor H concentrate.

摘要翻译： 用于从补体因子H纯化补体因子H的方法，所述补体因子H含有血液或血浆中的源，特别是含H因子的来源的辛酸盐沉淀物。 通过向血液或血浆的级分中加入辛酸盐离子获得，包括以下步骤：a）提供含有因子H的来源，特别是辛酸沉淀重构以提供补体因子H的溶液; b）特别是进行阳离子交换层析作为第一色谱步骤; c）进行阴离子交换层析; d）进行羟基磷灰石层析; e），然后进行超/渗滤以获得补体因子H浓缩物。