公开(公告)号：US10976247B2

公开(公告)日：2021-04-13

申请号：US16535330

申请日：2019-08-08

申请人： OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Liangxiao Zhang ,  Peiwu Li ,  Zhe Yuan ,  Du Wang ,  Xuefang Wang ,  Wen Zhang ,  Qi Zhang

IPC分类号： G01N21/359 ,  A01H5/10 ,  G01N33/03

摘要： The invention discloses a NIR method for fatty acid content of oilseeds, including: selection of oilseed samples, and analyzing the oilseed samples by an near infrared spectrometer to obtain NIR spectra; preprocessing of NIR spectra and establishment of a NIR spectral database of oilseeds; establishment of a fatty acid database of oilseeds based on gas chromatography; establishment of prediction model of fatty acids in oilseeds; acquiring NIR spectrum of a sample to be tested by the near infrared spectrometer, and importing the preprocessed NIR spectrum into the prediction model of fatty acids to obtain the predicted fatty acid content of the tested sample. The method is simple and rapid to operate and is non-destructive, and the detection time of the sample is greatly shortened and the detection cost is reduced.

公开(公告)号：US11098278B2

公开(公告)日：2021-08-24

申请号：US16594081

申请日：2019-10-07

申请人： OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Xiupin Wang ,  Peiwu Li ,  Huali Xie ,  Xuefang Wang ,  Qi Zhang ,  Xiaoxia Ding ,  Wen Zhang ,  Liangxiao Zhang

IPC分类号： C12N1/06 ,  C12N1/14 ,  C12N1/00 ,  G01N1/42

摘要： The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.