公开(公告)号：US20210046476A1

公开(公告)日：2021-02-18

申请号：US17010180

申请日：2020-09-02

Applicant: OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL CORPORATION

Inventor： Amy Shen FRIED ,  Sebastien Georg Gabriel RICOULT

IPC: B01L3/00 ,  C12M1/00 ,  G01N33/543 ,  G01N33/552

Abstract: It is an object of the present invention to achieve rapid surface patterning of biomolecules within microfluidic devices with high reproducibility. In this work, we present a new means of creating micro- and nano-patterns of aminosilanes within microfluidic devices via an aqueous based microcontact printing technique. To minimize the diffusion of molecules into the PDMS stamp, we use water as the inking solvent and enforce short incubation and contact times during the printing process to preserve the pre-defined resolution of patterned features. These patterns then serve as the building block to couple multiple biomolecules in solution onto a single surface for subsequent bioassays.

公开(公告)号：US20190030530A1

公开(公告)日：2019-01-31

申请号：US16073791

申请日：2017-02-01

Applicant: OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL CORPORATION

Inventor： Amy Shen FRIED ,  Sebastien Georg Gabriel RICOULT

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/552

Abstract: It is an object of the present invention to achieve rapid surface patterning of biomolecules within microfluidic devices with high reproducibility. In this work, we present a new means of creating micro- and nano-patterns of aminosilanes within microfluidic devices via an aqueous based microcontact printing technique. To minimize the diffusion of molecules into the PDMS stamp, we use water as the inking solvent and enforce short incubation and contact times during the printing process to preserve the predefined resolution of patterned features. These patterns then serve as the building block to couple multiple biomolecules in solution onto a single surface for subsequent bioassays.

公开(公告)号：US20180251717A1

公开(公告)日：2018-09-06

申请号：US15759734

申请日：2016-09-16

Applicant: OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL CORPORATION

Inventor： Hsieh-Fu TSAI ,  Amy Shen FRIED ,  Ji-Yen CHENG

IPC: C12M1/42 ,  C12M1/22

CPC classification number: C12M35/02 ,  C12M23/02 ,  C12M23/10

Abstract: A disclosed insert for a circular-shaped petri dish can generate a substantially uniform electric field across the petri dish that is filled with a fluid establishing a salt bridge. The insert includes a circular-shaped bottom plate defining a circular-shaped space; a side channel vertically erecting from a circular periphery of said bottom plate; and a pair of current rectifying chambers each having a generally planar shape communicating with the side channel. In at least some aspects of the invention, portions of the side channel bridging the pair of current rectifying chambers each have a generally concave top profile having a lowest point at the center between the pair of current rectifying chambers such that, when the salt bridge is established, the circular-shaped space defined by the bottom plate exhibits a substantially uniform electric field in a substantially entire area of the space.