公开(公告)号：US10982206B2

公开(公告)日：2021-04-20

申请号：US15972856

申请日：2018-05-07

Applicant: OLD DOMINION UNIVERSITY RESEARCH FOUNDATION

Inventor： Olga Pakhomova ,  Andrei G. Pakhomov

IPC: C12N13/00 ,  A61N1/32

Abstract: Methods of enhancing membrane permeabilization in a cell are provided. A method includes disposing the cell between a first electrode and a second electrode and applying a plurality of electrical pulses between the first electrode and the second electrode. In the method, the plurality of electrical pulses include at least two trains of pulses separated by an interval greater than about 10 s. Further, the amplitude of the electrical pulses is selected to be greater than about 0.2 kV/cm.

公开(公告)号：US20210214712A1

公开(公告)日：2021-07-15

申请号：US17215773

申请日：2021-03-29

Applicant: OLD DOMINION UNIVERSITY RESEARCH FOUNDATION

Inventor： Olga Pakhomova ,  Andrei G. Pakhomov

IPC: C12N13/00 ,  A61N1/32

Abstract: Systems and methods of enhancing membrane permeabilization in a cell are provided. An example method includes disposing the cell between a first electrode and a second electrode and applying a plurality of electrical pulses between the first electrode and the second electrode. In the systems and methods, the plurality of electrical pulses include at least two trains of pulses separated by an interval greater than about 10 s. Further, the amplitude of the electrical pulses is selected to be greater than about 0.2 kV/cm.

公开(公告)号：US11820976B2

公开(公告)日：2023-11-21

申请号：US17215773

申请日：2021-03-29

Applicant: OLD DOMINION UNIVERSITY RESEARCH FOUNDATION

Inventor： Olga Pakhomova ,  Andrei G. Pakhomov

IPC: A61N1/32 ,  C12N13/00

CPC classification number: C12N13/00 ,  A61N1/327

Abstract: Systems and methods of enhancing membrane permeabilization in a cell are provided. An example method includes disposing the cell between a first electrode and a second electrode and applying a plurality of electrical pulses between the first electrode and the second electrode. In the systems and methods, the plurality of electrical pulses include at least two trains of pulses separated by an interval greater than about 10 s. Further, the amplitude of the electrical pulses is selected to be greater than about 0.2 kV/cm.