公开(公告)号：US20230046749A1

公开(公告)日：2023-02-16

申请号：US17823685

申请日：2022-08-31

Applicant: OSAKA UNIVERSITY

Inventor： Hodaka FUJII ,  Toshitsugu FUJITA

IPC: C12Q1/6806 ,  C12N15/10 ,  G01N33/53

Abstract: A method comprising (A) fragmenting genomic DNA in a state where the interaction of the genomic DNA and molecules interacting therewith is maintained, and (B) bringing genomic DNA into contact with an exogenous molecule capable of binding to a specific endogenous DNA sequence in the genomic DNA. According to the method, with the use of an endogenous DNA sequence present inside or in the vicinity of a target genomic region in cells to be analyzed, any genomic region can be specifically isolated in a state where the interaction of the genomic region and molecules interacting therewith is maintained, without the need of inserting a recognition sequence of an exogenous DNA-binding molecule into the vicinity of the target genomic region in the cells to be analyzed.

公开(公告)号：US20160138081A1

公开(公告)日：2016-05-19

申请号：US14767068

申请日：2013-09-06

Applicant: OSAKA UNIVERSITY

Inventor： Hodaka FUJII ,  Toshitsugu FUJITA

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6806 ,  C12N15/1034 ,  C12Q1/6809 ,  G01N33/5308 ,  C12Q2522/101

Abstract: Provided is a method comprising (A) fragmenting genomic DNA in a state where the interaction of the genomic DNA and molecules interacting therewith is maintained, and (B) bringing genomic DNA into contact with an exogenous molecule capable of binding to a specific endogenous DNA sequence in the genomic DNA. According to the method, with the use of an endogenous DNA sequence present inside or in the vicinity of a target genomic region in cells to be analyzed, any genomic region can be specifically isolated in a state where the interaction of the genomic region and molecules interacting therewith is maintained, without the need of inserting a recognition sequence of an exogenous DNA-binding molecule into the vicinity of the target genomic region in the cells to be analyzed.

Abstract translation: 提供了一种方法，其包括（A）在维持基因组DNA和与其相互作用的分子的相互作用的状态下片段化基因组DNA，和（B）使基因组DNA与能够结合特异性内源DNA序列的外源分子接触 在基因组DNA中。 根据该方法，通过使用存在于要分析的细胞内的靶基因组区域内部或附近的内源性DNA序列，可以在基因组区域和分子的相互作用相互作用的状态下特异性分离任何基因组区域 保持，而不需要将外源DNA结合分子的识别序列插入待分析细胞中的靶基因组区域附近。