公开(公告)号：US06635474B1

公开(公告)日：2003-10-21

申请号：US09582490

申请日：2000-11-14

申请人： Ook Joon Yoo ,  Kyung Kwang Lee ,  Young Mahn Han ,  Sun Jung Kim ,  Hae Young Jeong ,  Jung Ho Ko ,  Won Jun Oh

发明人： Ook Joon Yoo ,  Kyung Kwang Lee ,  Young Mahn Han ,  Sun Jung Kim ,  Hae Young Jeong ,  Jung Ho Ko ,  Won Jun Oh

IPC分类号： C12N1563

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  C07K14/4732 ,  C07K14/535 ,  C12N15/63 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/85

摘要： There are disclosed mammary gland tissue-specific expression systems using the promoter site for the &bgr;-casein gene of Korean native goats, by use of which physiological activating substances can be produced. In each of the expression systems, that is, novel plasmids pGbc, pGbc_L and pGbc_S (deposition Nos. KCTC 0515BP, 0514BP and 0513BP, respectively), a &bgr;-casein gene expression-regulating region, a physiological activating substance gene and a termination-regulating region are linked. Human granulocyte colony stimulating factor (hG-CSF) or human granulocyte macrophage colony stimulating factor (hGM-CSF) can be produced in HC11 cells, a mouse mammary gland tissue-derived cell line, and in the milk secreted from the transgenic mice by use of a hG-CSF or hGM-CSF gene-carrying pGbc, pGbc_L or pGbc_S in transfection into cell and microinjection to mouse. The proteins are those which experience the posttranslational modification and maintain their normal activity in the human body. The expression systems make it possible to easily produce the proteins at a great amount, to scale up protein production to the extent of industrialization, and to isolate and purify the desired protein with ease and safety.

摘要翻译： 已经公开了使用韩国本地山羊的β-酪蛋白基因的启动子位点的乳腺组织特异性表达系统，通过使用可以产生生理活化物质。 在每个表达系统中，即新型质粒pGbc，pGbc_L和pGbc_S（分别为沉积编号KCTC 0515BP，0514BP和0513BP），β-酪蛋白基因表达调节区，生理活化物质基因和终止 - 调节区域相连。 人类粒细胞集落刺激因子（hG-CSF）或人粒细胞巨噬细胞集落刺激因子（hGM-CSF）可以在HC11细胞，小鼠乳腺组织来源的细胞系和转基因小鼠分泌的乳中产生 的hG-CSF或携带hGM-CSF基因的pGbc，pGbc_L或pGbc_S转染入细胞和显微注射到小鼠。 蛋白质是经历翻译后修饰并维持其在人体中的正常活性的蛋白质。 表达系统使得可以容易地大量生产蛋白质，将蛋白质生产扩大到工业化程度，并且容易且安全地分离和纯化所需的蛋白质。

公开(公告)号：US07053262B2

公开(公告)日：2006-05-30

申请号：US10347718

申请日：2003-01-17

申请人： Ook Joon Yoo ,  Kyung Kwang Lee ,  Young Mahn Han ,  Sun Jung Kim ,  Hae Young Jeong ,  Jung Ho Ko ,  Won Jun Oh

发明人： Ook Joon Yoo ,  Kyung Kwang Lee ,  Young Mahn Han ,  Sun Jung Kim ,  Hae Young Jeong ,  Jung Ho Ko ,  Won Jun Oh

IPC分类号： C12P21/00 ,  C12N15/63

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  C07K14/4732 ,  C07K14/535 ,  C12N15/63 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/85

摘要： The mammary gland-specific expression systems developed by the present inventors, named pGbc, pGbc_L and pGbc_S were deposited under the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure in the Korean Collection for Type Cultures (KCTC), Korean Research Institute of Bioscience and Biotechnology at 52, Oun-dong, Yusong-Ku, Taejon 305–333, Republic of Korea, on Aug. 17, 1998, and the accession deposit Nos. KCTC 0515BP, 0514BP and 0513BP were issued, respectively. All restructions on the availability to the public of the deposited materials will be irrevocably removed upon the granting of a patent. The systems of the present invention make it possible that desired proteins are produced by expression in mammary gland tissue-derived animal cells or through the milk secreted from the transgenic animals with the expression systems, thereby solving the above-mentioned problems, that is, the activity sustenance, production cost, and isolation and purification of the desired proteins.

摘要翻译： 由本发明人开发的命名为pGbc，pGbc_L和pGbc_S的本发明人的乳腺特异性表达系统根据布达佩斯条约保藏在国际识别为韩国专利文献收集专利程序中的微生物保藏（KCTC） 1998年8月17日，大韩民国Taejon 305-333 Ousong Ku，东京生物科技生物技术研究所52号，KCTC 0515BP，0514BP和0513BP登记号， 分别。 所有关于向公众提供存放资料的调整将在授予专利后不可撤销地删除。 本发明的系统使得可能通过在乳腺组织来源的动物细胞中表达所产生的蛋白质，或通过用表达系统从转基因动物分泌的乳中产生所需的蛋白质，由此解决上述问题，即， 活性维持，生产成本，以及所需蛋白质的分离和纯化。