公开(公告)号：US20160038903A1

公开(公告)日：2016-02-11

申请号：US14822540

申请日：2015-08-10

Applicant: PCL, INC.

Inventor： Minjoung Jo ,  Seram Lee

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00315 ,  B01J2219/0036 ,  B01J2219/00364 ,  B01J2219/00385 ,  B01J2219/00599 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00725

Abstract: A method of preparing a protein chip by gelation of a sol composition. In the method, a mixture of specific silicate monomers, such as SolB1, SolB2 and SolB3, SolBH, and a mixture of SolBS, distilled water and a detector protein are mixed sequentially, so that the gelation rate of the sol-composition can be delayed, thus inducing the stable gelation of the composition. Also, the biochip can be fabricated in a simple and easy manner by dispensing the sol composition by hand using an arrayer or a tool such as a pipette. In addition, a uniform biochip can be prepared by dispensing the sol composition, solution I (SolBH) and solution II (a mixture of buffer, SolBS, distilled water and a detector protein) sequentially onto a substrate without needing a conventional pretreatment process such as mixing.

Abstract translation: 一种通过凝胶化溶胶组合物制备蛋白质芯片的方法。 在该方法中，依次混合特定硅酸盐单体如SolB1，SolB2和SolB3，SolBH和SolBS，蒸馏水和检测器蛋白的混合物的混合物，使得溶胶组合物的凝胶化速率可以延迟 ，从而诱导组合物的稳定凝胶化。 此外，生物芯片可以通过使用排液器或诸如移液管的工具通过手动分配溶胶组合物以简单和容易的方式制造。 此外，可以通过将溶胶组合物溶液I（SolBH）和溶液II（缓冲液，SolBS，蒸馏水和检测器蛋白质的混合物）顺序分配到基底上而不需要常规的预处理过程例如 混合。