公开(公告)号：US20140162279A1

公开(公告)日：2014-06-12

申请号：US14071273

申请日：2013-11-04

申请人： PFENEX INC.

发明人： THOMAS M. RAMSEIER ,  HONGFAN JIN ,  CHARLES H. SQUIRES

IPC分类号： C12N15/63

CPC分类号： C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/78 ,  C12P21/02

摘要： This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.

摘要翻译： 本发明是改善重组蛋白或肽的生产水平或提高在宿主细胞中表达的活性重组蛋白或多肽的水平的方法。 本发明是比较表达重组蛋白的细胞的两种遗传图谱并修饰细胞以改变响应于重组蛋白表达上调的基因产物的表达的过程。 该方法可以改善蛋白质产生或者可以通过增加重组蛋白质的溶解度来改善蛋白质质量。