Abstract:
A chromatographic separating apparatus and method has at least a first chromatographic column having a separating material therein, having a length and is positioned in a first temperature zone maintained at a temperature (T1) and a second chromatographic column, serially connected with the first column, having the separating material therein having a length and is positioned in a second temperature zone maintained at a temperature (T2) which is of a different value than the first temperature (T1). Means are provided for passing sample fluid into the first column. The column is adapted to separate at least one preselected component from the fluid sample which comprises a multiplicity of components.