公开(公告)号：US20230091338A1

公开(公告)日：2023-03-23

申请号：US17904722

申请日：2021-02-22

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： AJITH ANAND ,  MAREN L ARLING ,  PIERLUIGI BARONE ,  HYEON-JE CHO ,  WILLIAM JAMES GORDON-KAMM ,  SANDEEP KUMAR ,  BRIAN LENDERTS ,  KEITH S LOWE ,  SERGEI SVITASHEV ,  XINLI EMILY WU

IPC分类号： C12N15/82 ,  A01H6/54 ,  A01H6/46 ,  C12N9/22 ,  C12N15/10

摘要： Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.