公开(公告)号：US20220124998A1

公开(公告)日：2022-04-28

申请号：US17571428

申请日：2022-01-07

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： AJITH ANAND ,  MAREN L ARLING ,  ALEXANDRE DA SILVA CONCEICAO ,  WILLIAM JAMES GORDON-KAMM ,  CRAIG HASTINGS ,  GEORGE J HOERSTER ,  TODD J JONES ,  THEODORE M KLEIN ,  CARLOS M LA ROTA ,  KEITH S LOWE ,  SHIV BAHADUR TIWARI ,  NING WANG ,  XINLI EMILY WU

IPC分类号： A01H4/00 ,  C12N15/82

摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.