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公开(公告)号：US20200270622A1
公开(公告)日：2020-08-27
申请号：US16650334
申请日：2018-09-19
发明人： ALEXANDRE DA SILVA CONCEICAO , ALLISON M FINCH , WILLIAM JAMES GORDON-KAMM , THEODORE M KLEIN , KEITH S LOWE
IPC分类号： C12N15/82
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include tissue-preferred promoters operably linked to morphogenic genes. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein operably linked to a morphogenic gene. DNA constructs comprising a promoter operably linked to a morphogenic gene and further comprising a heterologous nucleotide sequence of interest are also provided.
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2.发明公开公开(公告)号：US20230265448A1
公开(公告)日：2023-08-24
申请号：US18047714
申请日：2022-10-19
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE M KLEIN , KEITH S LOWE , KEVIN E MCBRIDE , CHRISTOPHER J SCELONGE , NING WANG
IPC分类号： C12N15/82
CPC分类号： C12N15/8241 , C12N15/8213 , C12N15/8216 , C12N15/8262
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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公开(公告)号：US20210277409A1
公开(公告)日：2021-09-09
申请号：US17255132
申请日：2019-06-25
发明人： AJITH ANAND , HYEON-JE CHO , THEODORE M KLEIN
IPC分类号： C12N15/82
摘要： Spectinomycin resistant or streptomycin resistant transgenic plants and methods of making such plants are provided.
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公开(公告)号：US20220124998A1
公开(公告)日：2022-04-28
申请号：US17571428
申请日：2022-01-07
发明人： AJITH ANAND , MAREN L ARLING , ALEXANDRE DA SILVA CONCEICAO , WILLIAM JAMES GORDON-KAMM , CRAIG HASTINGS , GEORGE J HOERSTER , TODD J JONES , THEODORE M KLEIN , CARLOS M LA ROTA , KEITH S LOWE , SHIV BAHADUR TIWARI , NING WANG , XINLI EMILY WU
摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
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5.发明申请公开(公告)号：US20210180077A1
公开(公告)日：2021-06-17
申请号：US17181136
申请日：2021-02-22
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE M KLEIN , KEITH S LOWE , KEVIN E MCBRIDE , CHRISTOPHER J SCELONGE , NING WANG
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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