公开(公告)号：US11015206B2

公开(公告)日：2021-05-25

申请号：US16404835

申请日：2019-05-07

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Phillip A Patten ,  Daniel Siehl ,  Yumin Tao ,  Henrik Albert ,  Ericka Bermudez ,  Linda A Castle ,  Yuxia Dong ,  Andrew Duncan Satterfield

IPC分类号： C12N15/82 ,  C12N9/02 ,  C12N15/52

摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

公开(公告)号：US11549123B2

公开(公告)日：2023-01-10

申请号：US16816349

申请日：2020-03-12

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Yuxia Dong ,  Michael Lassner ,  Jian Lu ,  Emily Ng ,  Phillip A Patten ,  Daniel Siehl

IPC分类号： C12N15/82 ,  C12N9/10

摘要： Compositions and methods comprising polynucleotides and polypeptides having EPSP (5-enolpyruvylshikimate-3-phosphate) synthase (EPSPS) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, improved catalytic capacity in the presence of the inhibitor, glyphosate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the EPSPS sequences. Various methods of employing the EPSPS sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

公开(公告)号：US10655141B2

公开(公告)日：2020-05-19

申请号：US15764388

申请日：2016-09-29

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Yuxia Dong ,  Michael Lassner ,  Jian Lu ,  Emily Ng ,  Phillip A. Patten ,  Daniel Siehl

IPC分类号： C12N15/82 ,  C12N9/10

摘要： Compositions and methods comprising polynucleotides and polypeptides having EPSP (5-enolpyruvylshikimate-3-phosphate) synthase (EPSPS) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, improved catalytic capacity in the presence of the inhibitor, glyphosate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the EPSPS sequences. Various methods of employing the EPSPS sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

公开(公告)号：US10316326B2

公开(公告)日：2019-06-11

申请号：US15305457

申请日：2015-05-02

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Daniel Siehl ,  Phillip A. Patten ,  Yumin Tao ,  Ericka Bermudez ,  Linda A. Castle ,  Yuxia Dong ,  Andrew Duncan Satterfield ,  Henrik Albert

IPC分类号： C12N15/82 ,  C12N9/02 ,  C12N15/52

摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

公开(公告)号：US20170058291A1

公开(公告)日：2017-03-02

申请号：US15305457

申请日：2015-05-02

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Daniel Siehl ,  Phillip A. Patten ,  Yumin Tao ,  Ericka Bermudez ,  Linda A. Castle ,  Yuxia Dong ,  Andrew Duncan Satterfield ,  Henrik Albert

IPC分类号： C12N15/82 ,  C12N15/52 ,  C12N9/02

CPC分类号： C12N15/8274 ,  C12N9/0069 ,  C12N15/52 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8278 ,  C12Y113/11027

摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

摘要翻译： 提供了包含具有4-羟基苯基丙酮酸双加氧酶（HPPD）活性并且对HPPD抑制剂不敏感的多核苷酸和多肽的组合物和方法。 还提供了具有HPPD序列的核酸构建体，植物，植物细胞，外植体，种子和谷粒。 提供了使用HPPD序列的各种方法。 这样的方法包括例如用于产生HPPD抑制剂耐受植物，植物细胞，外植体或种子的方法以及在含有本文公开的植物和/或种子的作物的田地中控制杂草的方法。 还提供了用于鉴定另外的HPPD变体的方法。 进一步提供了允许各种HPPD多肽及其变体和片段在叶绿体中表达或转运至叶绿体的各种方法和组合物。