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公开(公告)号：US20160025697A1
公开(公告)日：2016-01-28
申请号：US14776158
申请日：2014-03-10
IPC分类号： G01N33/00
CPC分类号： G01N33/0098 , A01H1/04
摘要： Methods are provided for evaluating the insect resistance of a plant of interest. Specifically, methods are provided for high-throughput screening of soybean plants for resistance to kudzu bugs and stink bugs, including the brown marmorated stink bug and the southern green stink bug. In some embodiments, infesting emergent soybean seedlings with second instar stink bug nymphs and maintaining the nymphs in a closed environment with the seedling for only about 7 days allows evaluation of the stink bug resistance of a soybean variety of interest. Also provided are insect resistant plants, particularly plants resistant to kudzu bugs and stink bugs, as well as seeds produced by the resistant plants. Plants disclosed herein can be used to transfer the resistant trait into plant lines of interest.
摘要翻译： 提供了评估感兴趣植物的抗虫性的方法。 具体来说，提供了大豆植物高通量筛选方法，用于抵抗葛虫和臭臭虫，包括棕色马蹄臭虫和南方绿色臭虫。 在一些实施方案中，具有第二龄臭蝽若虫的侵袭性大豆幼苗并且在封闭环境中将幼苗保持在约7天的幼苗中，可以评估所关心的大豆品种的臭椿抗性。 还提供了抗虫植物，特别是抗葛虫和臭虫的植物，以及由抗性植物产生的种子。 本文公开的植物可以用于将抗性性状转移到感兴趣的植物系中。
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公开(公告)号：US20130174295A1
公开(公告)日：2013-07-04
申请号：US13724154
申请日：2012-12-21
IPC分类号： C12N15/82
CPC分类号： A01H1/04 , A01H1/00 , A01H1/02 , A01H5/10 , C12N15/8286 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： This invention relates to methods of improving resistance to aphids in soybean plants, as well as methods for identifying and/or selecting soybean plants or germplasm that display improved resistance to one or more biotypes of soybean aphid. In certain examples, the method comprises selecting a first and second soybean plant or germplasm, each of which has a different favorable Rag1, Rag2, or Rag3, allele, haplotype, or marker profile, and crossing those first and second soybean plants to produce a progeny plant with improved soybean aphid resistance. This invention further relates to markers, primers, probes, kits, systems, etc., useful for carrying out the methods described herein.
摘要翻译： 本发明涉及改善大豆植物中对蚜虫抗性的方法，以及用于鉴定和/或选择显示对一种或多种大豆蚜虫生物型的改良抗性的大豆植物或种质的方法。 在某些实例中，该方法包括选择第一种和第二种大豆植物或种质，每种大豆植物或种质均具有不同的有利的Rag1，Rag2或Rag3等位基因，单倍型或标记特征，并与第一和第二大豆植物杂交以产生 具有改良大豆蚜虫抗性的后代植物。 本发明还涉及可用于实施本文所述方法的标记，引物，探针，试剂盒，系统等。
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3.发明授权公开(公告)号：US09284574B2
公开(公告)日：2016-03-15
申请号：US13724154
申请日：2012-12-21
CPC分类号： A01H1/04 , A01H1/00 , A01H1/02 , A01H5/10 , C12N15/8286 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： This invention relates to methods of improving resistance to aphids in soybean plants, as well as methods for identifying and/or selecting soybean plants or germplasm that display improved resistance to one or more biotypes of soybean aphid. In certain examples, the method comprises selecting a first and second soybean plant or germplasm, each of which has a different favorable Rag1, Rag2, or Rag3, allele, haplotype, or marker profile, and crossing those first and second soybean plants to produce a progeny plant with improved soybean aphid resistance. This invention further relates to markers, primers, probes, kits, systems, etc., useful for carrying out the methods described herein.
摘要翻译： 本发明涉及改善大豆植物中对蚜虫抗性的方法，以及用于鉴定和/或选择显示对一种或多种大豆蚜虫生物型的改良抗性的大豆植物或种质的方法。 在某些实例中，该方法包括选择第一种和第二种大豆植物或种质，每种大豆植物或种质均具有不同的有利的Rag1，Rag2或Rag3等位基因，单倍型或标记特征，并与第一和第二大豆植物杂交以产生 具有改良大豆蚜虫抗性的后代植物。 本发明还涉及可用于实施本文所述方法的标记，引物，探针，试剂盒，系统等。
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