公开(公告)号：US20230256449A1

公开(公告)日：2023-08-17

申请号：US17996982

申请日：2021-04-28

Applicant: POLITECNICO DI MILANO ,  FONDAZIONE CENTRO SAN RAFFAELE

Inventor： Elena BIANCHI ,  Oronza Antonietta BOTRUGNO ,  Gabriele Angelo DUBINI ,  Monica PIERGIOVANNI ,  Giovanni TONON ,  Paola DE STEFANO ,  Luca LEONI ,  Pietro OLDANI

IPC: B01L3/00

CPC classification number: B01L3/5088 ,  B01L3/5085 ,  B01L2300/161 ,  B01L2300/0829

Abstract: A micro-droplet plate including at least one fixed element which is a rigid flat support which has an upper face and a lower face, having, on said upper face, wells connected together by microchannels, said wells being delimited by a hydrophobic rim and crossed by a hole, where said wells and said microchannels define at least one microfluidic circuit; and moving elements, received in said wells through said holes, which are cylinders having an upper base and a lower base. The moving elements move, even independently of each other, along their vertical axis, and the upper base of said moving elements includes a hydrophilic surface with relief items.

公开(公告)号：US20220257844A1

公开(公告)日：2022-08-18

申请号：US17619411

申请日：2020-06-19

Applicant: POLITECNICO DI MILANO

Inventor： Maria Laura COSTANTINO ,  Giustina CASAGRANDE ,  Monica PIERGIOVANNI ,  Elena BIANCHI ,  Clara BERNARDELLI

IPC: A61M1/30 ,  C12N5/078 ,  A61M1/02 ,  A01N1/02 ,  A61M1/36

Abstract: It is an object of the present invention a method to introduce compounds inside red blood cells comprising: —providing red blood cells from a subject; —providing one or more compounds to be encapsulated in said red blood cells; —providing a loading device comprising a microporous matrix; —feeding said loading device with a suspension comprising said red blood cells and said one or more compounds; —collecting the red blood cells exiting from said loading device, which are encapsulated red blood cells; characterized in that: —said red blood cells and said one or more compound are in suspension at a pH of between 6.8 and 7.8, preferably of between 7.35 and 7.45; —the pores in said microporous matrix have a minimum size of at least 3 times the size of a red blood cell, that is a minimum size of at least 20 μm; —the pores in said microporous matrix have an average size of between 30 and 500 μm, or of between 40 and 400 μm, or of between 50 and 350 μm, or of between 100 and 250 μm; —said porous matrix has a length L of at least 1 mm and a width W and a height H such that it comprises at least one unit cell, said unit cell being equal to the microporous matrix volume that, repeated by rototranslation through the vectors that generate the matrix, fills the whole matrix itself; wherein said loading device is fed with said suspension in such a way to obtain an average fluid speed of between 10−4 and 10 m/s. Further objects of the present invention are a microporous matrix, a loading device comprising the same, a fluidic circuit and a machine for implementing said method and red blood cells encapsulated with at least one compound according to said method.