公开(公告)号：US11693016B2

公开(公告)日：2023-07-04

申请号：US16698872

申请日：2019-11-27

申请人： POLYMER TECHNOLOGY SYSTEMS, INC.

发明人： Donnie Smith ,  Brittney L. Werner ,  Gary L. Hughes ,  Jie Chen

IPC分类号： G01N33/72 ,  G01N27/327 ,  C12N9/50

CPC分类号： G01N33/721 ,  C12N9/50 ,  G01N27/3272 ,  G01N33/723

摘要： A method for determining analytes includes lysing the red blood cells of a whole blood sample, oxidizing the free hemoglobin in the lysed sample, and cleaving FVH from the hemoglobin A1C to form an electrochemical test solution. In one aspect, a first portion of the electrochemical test solution is reacted with fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product. A first electrical property of the first reaction product is measured, the measurement being indicative of hemoglobin A1C in the blood sample. In another aspect, a second portion of the electrochemical test solution is reacted with ferrocyanide to form a second reaction product. A second electrical property of the second reaction product is measured, the measurement being indicative of total hemoglobin in the blood sample. Hemoglobin A1C, total hemoglobin, and % HbA1C are determined based on the first and second electrical properties.

公开(公告)号：US20230305026A1

公开(公告)日：2023-09-28

申请号：US18328942

申请日：2023-06-05

申请人： POLYMER TECHNOLOGY SYSTEMS, INC.

发明人： Donnie Smith ,  Brittney L. Werner ,  Gary L. Hughes ,  Jie Chen

IPC分类号： G01N33/72 ,  C12N9/50 ,  G01N27/327

CPC分类号： G01N33/721 ,  C12N9/50 ,  G01N27/3272 ,  G01N33/723

摘要： A method is disclosed comprising lysing the red blood cells of a whole blood sample, oxidizing the free hemoglobin in the lysed sample, and cleaving FVH from the hemoglobin A1C to form an electrochemical test solution. In one aspect, a first portion of the electrochemical test solution is reacted with fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product. A first electrical property of the first reaction product is measured, the measurement being indicative of hemoglobin A1C in the blood sample. In another aspect, a second portion of the electrochemical test solution is reacted with ferrocyanide to form a second reaction product. A second electrical property of the second reaction product is measured, the measurement being indicative of total hemoglobin in the blood sample. Hemoglobin A1C, total hemoglobin, and % HbA1C are determined based on the first and second electrical properties. Also provided are systems and components useful in performing the disclosed methods.

公开(公告)号：US20200174024A1

公开(公告)日：2020-06-04

申请号：US16698872

申请日：2019-11-27

申请人： POLYMER TECHNOLOGY SYSTEMS, INC.

发明人： Donnie Smith ,  Brittney L. Werner ,  Gary L. Hughes ,  Jie Chen

IPC分类号： G01N33/72 ,  G01N27/327 ,  C12N9/50

摘要： A method is disclosed comprising lysing the red blood cells of a whole blood sample, oxidizing the free hemoglobin in the lysed sample, and cleaving FVH from the hemoglobin A1C to form an electrochemical test solution. In one aspect, a first portion of the electrochemical test solution is reacted with fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product. A first electrical property of the first reaction product is measured, the measurement being indicative of hemoglobin A1C in the blood sample. In another aspect, a second portion of the electrochemical test solution is reacted with ferrocyanide to form a second reaction product. A second electrical property of the second reaction product is measured, the measurement being indicative of total hemoglobin in the blood sample. Hemoglobin A1C, total hemoglobin, and % HbA1C are determined based on the first and second electrical properties. Also provided are systems and components useful in performing the disclosed methods.

公开(公告)号：US11852604B2

公开(公告)日：2023-12-26

申请号：US16913704

申请日：2020-06-26

申请人： Polymer Technology Systems, Inc.

发明人： Jie Chen ,  Christopher Dailey ,  Gary L. Hughes

IPC分类号： G01N27/327

CPC分类号： G01N27/3272 ,  G01N27/3273 ,  G01N27/3274

摘要： In one embodiment, a system for detecting an analyte in a bodily fluid sample includes an electrochemical biosensor, the electrochemical biosensor configured to receive the bodily fluid sample, test for the analyte, and produce an analyte level for the analyte. The system further includes a mediator, the mediator configured to provide for the testing of the analyte by facilitating electron exchange between the analyte and the biosensor. The system further includes an oxygen tension reducing agent, the oxygen tension reducing agent reducing interference of oxygen with the operation of the mediator.