公开(公告)号：US20220162690A1

公开(公告)日：2022-05-26

申请号：US17495739

申请日：2021-10-06

Applicant: Pacific Biosciences of California, Inc.

Inventor： Kandaswamy VIJAYAN ,  Corey M. DAMBACHER ,  Eugene TU ,  Mark A. BERNARD ,  Joseph ROKICKI ,  Kerry WILSON

IPC: C12Q1/6869 ,  C12Q1/6874 ,  C12N15/10 ,  C12P19/34 ,  G01N33/50

Abstract: Provided are compositions, methods and systems for determining the sequence of a template nucleic acid using a polymerase-based, sequencing-by-binding procedure. An examination step involves monitoring the interaction between a polymerase and template nucleic acid in the presence of one or more nucleotides. Identity of the next correct nucleotide in the sequence is determined without incorporation of any nucleotide into the structure of the primer by formation of a phosphodiester bond. An optional incorporation step can be used after the examination step to extend the primer by one or more nucleotides, thereby incrementing the template nucleotides that can be examined in a subsequent examination step. The sequencing-by-binding procedure does not require the use of labeled nucleotides or polymerases, but optionally can employ these reagents.

公开(公告)号：US20230151420A1

公开(公告)日：2023-05-18

申请号：US17876893

申请日：2022-07-29

Applicant: Pacific Biosciences of California, Inc.

Inventor： Sean STROMBERG ,  John VIECELI ,  Kandaswamy VIJAYAN ,  Arnold OLIPHANT

IPC: C12Q1/6874 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6869

CPC classification number: C12Q1/6874 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6869

Abstract: A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.

公开(公告)号：US20240035082A1

公开(公告)日：2024-02-01

申请号：US18045687

申请日：2022-10-11

Applicant: Pacific Biosciences of California, Inc.

Inventor： Kandaswamy VIJAYAN ,  Eugene TU ,  Mark A. BERNARD

IPC: C12Q1/6869

CPC classification number: C12Q1/6869

Abstract: The present disclosure provides compositions, methods and systems for sequencing a template nucleic acid using a polymerase based, nucleic acid binding reaction involving examination of the interaction between a polymerase and template nucleic acid in the presence of one or more unlabeled nucleotides. The methods rely, in part, on identifying a base of a template nucleic acid during nucleic acid synthesis by controlling the sequencing reaction conditions. Template nucleic acid bases may be identified during an examination step followed by an optional incorporation step.