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公开(公告)号：US09109193B2
公开(公告)日：2015-08-18
申请号：US12124039
申请日：2008-05-20
CPC分类号： C12M29/10 , B01D29/13 , C12M23/14 , C12M23/26 , C12M23/48 , C12M27/02 , C12M27/20 , C12M29/00 , C12M29/18 , C12M47/10
摘要： Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein.
摘要翻译： 提供了用于容纳和操纵液体的系统和方法，包括容器和单元操作或细胞培养物，细胞容纳物，生物反应器和/或药物制造系统的组分。 在某些实施方案中，这样的容器和单元操作涉及连续灌注反应器或生物反应器系统，并且可以包括一个或多个一次性组件。 例如，一方面，系统包括生物反应器形式的装置，用于收集产生一种或多种产品的细胞。 该装置可以包括适于容纳液体的一次性可折叠袋，该可折叠袋与液体 - 固体（例如，池）分离装置流体连通。 例如，可折叠袋的出口可以连接到分离装置的入口，并且分离装置的出口可以连接到可折叠袋的入口以用于再循环。 因此，在分离装置中的液体分离细胞后，细胞可以流回到可收缩的袋子中，在那里它们可以被再次收获。 同时，包含在液体中的产品可以收集在单独的容器中。 通过使用本文所述的混合系统可以提高这种系统中产物形成的效率。
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公开(公告)号：US20090035856A1
公开(公告)日：2009-02-05
申请号：US12124039
申请日：2008-05-20
CPC分类号： C12M29/10 , B01D29/13 , C12M23/14 , C12M23/26 , C12M23/48 , C12M27/02 , C12M27/20 , C12M29/00 , C12M29/18 , C12M47/10
摘要： Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein.
摘要翻译： 提供了用于容纳和操纵液体的系统和方法，包括容器和单元操作或细胞培养物，细胞容纳物，生物反应器和/或药物制造系统的组分。 在某些实施方案中，这样的容器和单元操作涉及连续灌注反应器或生物反应器系统，并且可以包括一个或多个一次性组件。 例如，一方面，系统包括生物反应器形式的装置，用于收集产生一种或多种产品的细胞。 该装置可以包括适于容纳液体的一次性可折叠袋，该可折叠袋与液体 - 固体（例如，池）分离装置流体连通。 例如，可折叠袋的出口可以连接到分离装置的入口，并且分离装置的出口可以连接到可折叠袋的入口以用于再循环。 因此，在分离装置中的液体分离细胞后，细胞可以流回到可收缩的袋子中，在那里它们可以被再次收获。 同时，包含在液体中的产品可以收集在单独的容器中。 通过使用本文所述的混合系统可以提高这种系统中产物形成的效率。
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