公开(公告)号：US20210236646A1

公开(公告)日：2021-08-05

申请号：US17049208

申请日：2019-04-25

申请人： Patrick Leonard AHL ,  Akhilesh BHAMBHANI ,  Christopher Jon FARRELL, ,  Patrick MHUGH ,  Morrisa C. JONES ,  Daniel D. ROTH ,  Jessica R. SINACOLA ,  Justin STANBRO ,  Matthew P. WATSON ,  Emily WEN ,  Michael A. WINTERS ,  MERCK SHARP & DOHME CORP.

发明人： Patrick Leonard AHL ,  Akhilesh BHAMBHANI ,  Christopher Jon FARRELL ,  Patrick McHUGH ,  Morrisa C. JONES ,  Daniel D. ROTH ,  Jessica R. SINACOLA ,  Justin STANBRO ,  Matthew P. WATSON ,  Emily WEN ,  Michael A. WINTERS

IPC分类号： A61K47/61 ,  A61K39/09

摘要： A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized in the form of lyospheres of carrier protein and lyospheres of the one or more polysaccharides. A predetermined amount of carrier protein lyospheres and activated polysaccharide lyospheres are mixed together and the mixture reconstituted in an organic solvent to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.