公开(公告)号：US20210252125A1

公开(公告)日：2021-08-19

申请号：US17049245

申请日：2019-04-25

申请人： Patrick Leonard AHL ,  Akhilesh BHAMBHANI ,  Christopher Jon FARRELL ,  Patrick MCHUGH ,  Morrisa C. JONES ,  Daniel D. ROTH ,  Jessica R. SINACOLA ,  Justin STANBRO ,  Matthew P. WATSON ,  Emily WEN ,  Michael A. WINTERS ,  Merck Sharp & Dohme Corp.

发明人： Patrick Leonard Ahl ,  Akhilesh Bhambhani ,  Christopher Jon Farrell ,  Patrick McHugh ,  Morrisa C. Jones ,  Daniel D. Roth ,  Jessica R. Sinacola ,  Justin Stanbro ,  Matthew P. Watson ,  Emily Wen ,  Michael A. Winters

IPC分类号： A61K39/09 ,  A61K47/62 ,  A61K47/61

摘要： A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.

公开(公告)号：US20210236646A1

公开(公告)日：2021-08-05

申请号：US17049208

申请日：2019-04-25

申请人： Patrick Leonard AHL ,  Akhilesh BHAMBHANI ,  Christopher Jon FARRELL, ,  Patrick MHUGH ,  Morrisa C. JONES ,  Daniel D. ROTH ,  Jessica R. SINACOLA ,  Justin STANBRO ,  Matthew P. WATSON ,  Emily WEN ,  Michael A. WINTERS ,  MERCK SHARP & DOHME CORP.

发明人： Patrick Leonard AHL ,  Akhilesh BHAMBHANI ,  Christopher Jon FARRELL ,  Patrick McHUGH ,  Morrisa C. JONES ,  Daniel D. ROTH ,  Jessica R. SINACOLA ,  Justin STANBRO ,  Matthew P. WATSON ,  Emily WEN ,  Michael A. WINTERS

IPC分类号： A61K47/61 ,  A61K39/09

摘要： A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized in the form of lyospheres of carrier protein and lyospheres of the one or more polysaccharides. A predetermined amount of carrier protein lyospheres and activated polysaccharide lyospheres are mixed together and the mixture reconstituted in an organic solvent to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.

公开(公告)号：US20210223262A1

公开(公告)日：2021-07-22

申请号：US15734346

申请日：2019-06-03

申请人： Akhilesh BHAMBHANI ,  Thorsten VERCH ,  Morrisa C. JONES ,  Mary RETZLAFF ,  Jessica R. SINACOLA ,  Silikhone L. BOUARAPHAN ,  Merck Sharp & Dohme Corp.

发明人： Akhilesh Bhambhani ,  Thorsten Verch ,  Morrisa C. Jones ,  Mary Retzlaff ,  Jessica R. Sinacola ,  Silikhone L. Bouaraphan

IPC分类号： G01N33/68 ,  G01N33/58

摘要： The present invention provides lyophilized pellets of low concentrations of antibodies in the form of lyospheres. These lyospheres provide pre-measured antibody concentrations suitable for diagnostic and quality control assays.