公开(公告)号：US20070212699A1

公开(公告)日：2007-09-13

申请号：US11518353

申请日：2006-09-08

申请人： Peter Laird ,  Cindy Eads ,  Kathleen Danenberg

发明人： Peter Laird ,  Cindy Eads ,  Kathleen Danenberg

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q2523/125

摘要： There is disclosed an improved high-throughput and quantitative process for determining methylation patterns in genomic DNA samples based on amplifying modified nucleic acid, and detecting methylated nucleic acid based on amplification-dependent displacement of specifically annealed hybridization probes. Specifically, the inventive process provides for treating genomic DNA samples with sodium bisulfite to create methylation-dependent sequence differences, followed by detection with fluorescence-based quantitative PCR techniques. The process is particularly well suited for the rapid analysis of a large number of nucleic acid samples, such as those from collections of tumor tissues

摘要翻译： 公开了基于扩增修饰的核酸来确定基因组DNA样品中的甲基化模式的改进的高通量和定量方法，并且基于特异性退火的杂交探针的扩增依赖位移来检测甲基化的核酸。 具体地，本发明方法提供用亚硫酸氢钠处理基因组DNA样品以产生甲基化依赖性序列差异，随后用基于荧光的定量PCR技术进行检测。 该方法特别适用于大量核酸样品的快速分析，例如来自肿瘤组织的核酸样品