公开(公告)号：US5652339A

公开(公告)日：1997-07-29

申请号：US361132

申请日：1994-12-21

申请人： Peter Lerch ,  Gerhard Hodler ,  Vreni Fortsch

发明人： Peter Lerch ,  Gerhard Hodler ,  Vreni Fortsch

IPC分类号： A61K38/00 ,  A61K9/127 ,  A61P3/06 ,  C07K1/22 ,  C07K14/775 ,  C07K1/00 ,  C07K14/00

CPC分类号： C07K14/775 ,  A61K9/1275

摘要： Reconstituted high density lipoproteins (rHDL) are produced from apolipoproteins and lipids in that an aqueous apolipoprotein solution with a concentration of 1-40 g protein/l is mixed with an aqueous lipid-detergent solution without mandatory addition of organic solvents, the molar ratio of lipid to detergent being 1:0.5 to 1:4 and the weight ratio of lipid to detergent being 1:1.5 to 1:5, the resultant apolipoprotein-lipid-detergent mixture then being incubated at a temperature in the range of the phase change temperature of the lipid in water .+-.10.degree. C., and the detergent being at least partially removed.The rHDL are useful for various prophylactic and therapeutic treatments of diseases that have a connection with lipids and lipidoidal substances. This method is especially suitable for technical and industrial production.

摘要翻译： 重组的高密度脂蛋白（rHDL）由载脂蛋白和脂质产生，因为浓度为1-40g蛋白/ l的载脂蛋白溶液溶液与水性脂质 - 洗涤剂溶液混合，而不必强制加入有机溶剂，摩尔比 脂类与洗涤剂的比例为1:0.5至1:4，脂质与洗涤剂的重量比为1:1.5至1:5，然后将所得载脂蛋白脂质洗涤剂混合物在相变温度范围内的温度 的脂质在水中+/- 10℃，并且洗涤剂被至少部分去除。 rHDL可用于与脂质和脂质物质有关联的疾病的各种预防和治疗性治疗。 这种方法特别适用于技术和工业生产。