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    Seed shattering
    2.
    发明授权
    Seed shattering 失效
    种子粉碎

    公开(公告)号：US06420628B1

    公开(公告)日：2002-07-16

    申请号：US09051239

    申请日：1998-09-28

    申请人： Peter Ulvskov ,  Robin Child ,  Henri Van Onckelen ,  Els Prinsen ,  Bernhard Borkhardt ,  Lilli Sander ,  Morten Petersen ,  Gert Bundgard Poulsen ,  Johan Botterman

    发明人： Peter Ulvskov ,  Robin Child ,  Henri Van Onckelen ,  Els Prinsen ,  Bernhard Borkhardt ,  Lilli Sander ,  Morten Petersen ,  Gert Bundgard Poulsen ,  Johan Botterman

    IPC分类号： C12N1509

    CPC分类号： C12N15/8249 ,  C12N9/2402 ,  C12N15/8206 ,  C12Y302/01015

    摘要： A plant is provided which contains at least one dehiscence zone (DZ)-selective chimeric gene incorporated in the nuclear genome of its cells, wherein said DZ-selective chimeric gene comprises the following operably linked DNA fragments: a) a transcribed DNA region encoding: 1) a RNA which, when produced in cells of a particular DZ of the plant, prevents, inhibits or reduces the expression in DZ cells of an endogenous gene of the plant encoding a cell wall hydrolase, particularly an endopolygalacturonase, or, 2) a protein or polypeptide, which when produced in DZ cells, kills or disables them or interferes with their normal metabolism, physiology or development; b) a plant expressible promoter which directs expression of transcribed DNA region at least in DZ cells, provided that if transcribed DNA region encodes a protein or polypeptide, or encodes an antisense RNA or ribozyme directed to a sense RNA encoded by an endogenous gene that is expressed in plant in cells other than DZ cells, plant expressible promoter is a DZ-selective promoter, which directs expression of transcribed region selectively in DZ cells and wherein plant is characterized by modified dehiscence properties, preferably delayed dehiscence, when compared to a plant not containing DZ-selective chimeric gene.

    摘要翻译： 提供了一种植物，其包含并入其细胞的核基因组中的至少一个开裂区（DZ）选择性嵌合基因，其中所述DZ选择性嵌合基因包含以下可操作地连接的DNA片段：a）编码： 1）当在植物的特定DZ的细胞中产生的RNA在编码细胞壁水解酶，特别是内聚半乳糖醛酸酶的植物的内源基因的DZ细胞中预防，抑制或降低表达时，或者，2） 蛋白质或多肽，当在DZ细胞中产生时，它们杀死或消灭它们或干扰其正常代谢，生理或发育; b）至少在DZ细胞中指示转录的DNA区域的表达的植物可表达的启动子，条件是如果转录的DNA区编码蛋白质或多肽，或编码针对由内源基因编码的正义RNA的反义RNA或核酶， 在DZ细胞以外的细胞中植物表达，植物可表达的启动子是DZ选择性启动子，其指导转录区域在DZ细胞中的选择性表达，并且其中植物的特征在于与植物不相似的改性开裂性质，优选延迟开裂 含有DZ选择性嵌合基因。