公开(公告)号：US10689640B2

公开(公告)日：2020-06-23

申请号：US15384029

申请日：2016-12-19

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  Thomas M. Ramseier ,  Russell J. Coleman ,  Jane C. Schneider ,  Charles D. Hershberger

IPC分类号： C12N15/10 ,  C12N15/78

摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous recombinant protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous recombinant protein of interest in a periplasm compartment or may secrete the heterologous recombinant protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

公开(公告)号：US20170183646A1

公开(公告)日：2017-06-29

申请号：US15384029

申请日：2016-12-19

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  Thomas M. Ramseier ,  Russell J. Coleman ,  Jane C. Schneider ,  Charles D. Hershberger

IPC分类号： C12N15/10

摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.