公开(公告)号：US20180291412A1

公开(公告)日：2018-10-11

申请号：US16014348

申请日：2018-06-21

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  David C. Watkins ,  Stacey L. Lee ,  Frank H. Gaertner ,  Robert Shutter

IPC分类号： C12P21/00 ,  C07K14/61 ,  C07K14/52 ,  C12P21/02 ,  C12N15/78 ,  C07K16/40 ,  C07K14/57

摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.

公开(公告)号：US09109229B2

公开(公告)日：2015-08-18

申请号：US14071273

申请日：2013-11-04

申请人： Pfenex Inc.

发明人： Thomas M. Ramseier ,  Hongfan Jin ,  Charles H. Squires

IPC分类号： C12N15/67 ,  C12N15/63 ,  C12N15/65 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/78 ,  C12P21/02

CPC分类号： C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/78 ,  C12P21/02

摘要： This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.

摘要翻译： 本发明是改善重组蛋白或肽的生产水平或提高在宿主细胞中表达的活性重组蛋白或多肽的水平的方法。 本发明是比较表达重组蛋白的细胞的两种遗传图谱并修饰细胞以改变响应于重组蛋白表达上调的基因产物的表达的过程。 该方法可以改善蛋白质产生或者可以通过增加重组蛋白质的溶解度来改善蛋白质质量。

公开(公告)号：US10689640B2

公开(公告)日：2020-06-23

申请号：US15384029

申请日：2016-12-19

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  Thomas M. Ramseier ,  Russell J. Coleman ,  Jane C. Schneider ,  Charles D. Hershberger

IPC分类号： C12N15/10 ,  C12N15/78

摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous recombinant protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous recombinant protein of interest in a periplasm compartment or may secrete the heterologous recombinant protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

公开(公告)号：US10041102B2

公开(公告)日：2018-08-07

申请号：US15230192

申请日：2016-08-05

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  David C. Watkins ,  Stacey L. Lee ,  Frank H. Gaertner ,  Robert Shutter

IPC分类号： C12P21/02 ,  C12P21/06 ,  C07K14/52 ,  C12P21/00 ,  C07K16/40 ,  C12N15/78 ,  C07K14/57 ,  C07K14/61

摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.

公开(公告)号：US20170183646A1

公开(公告)日：2017-06-29

申请号：US15384029

申请日：2016-12-19

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  Thomas M. Ramseier ,  Russell J. Coleman ,  Jane C. Schneider ,  Charles D. Hershberger

IPC分类号： C12N15/10

摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

公开(公告)号：US20170051327A1

公开(公告)日：2017-02-23

申请号：US15230192

申请日：2016-08-05

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  David C. Watkins ,  Stacey L. Lee ,  Frank H. Gaertner ,  Robert Shutter

IPC分类号： C12P21/00 ,  C12N15/78 ,  C07K14/61 ,  C07K16/40 ,  C07K14/57

CPC分类号： C12P21/00 ,  C07K14/52 ,  C07K14/57 ,  C07K14/61 ,  C07K16/40 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/78 ,  C12P21/02 ,  C12Y302/01023

摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable Circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.

摘要翻译： 本发明是通过在假单胞菌，特别是荧光假单胞菌生物体中表达来改进重组哺乳动物蛋白质生产的方法。 本方法提供了哺乳动物蛋白质，特别是人或人衍生的蛋白质，通过已知的表达系统如大肠杆菌在可比较的情况下改进生产分离的哺乳动物，特别是人类蛋白质的方法。